AI Article Synopsis
- The study developed chitosan-based implants that can carry non-viral DNA vectors, showing compatibility with living tissues.
- The implants were tested on transgenic rats, specifically designed to visualize stem cells, by placing them into the rats' fat tissue.
- After 8 days, researchers observed cells expressing YFP fluorescence and markers associated with stemness, indicating successful gene transfection.
Article Abstract
The implants from natural polymer chitosan was developed by sorption of non-viral DNA vector. These assemblies were shown to be biocompatible and able to transfect the surrounding cells with reprogramming factors. Transgenic rats from the strain allowing visualization of Nanog-positive stem cells received these implants into their adipose deposits. After 8 days we were able to detect in situ-transfected cells. Transfected cells were positive for YFP fluorescence and transiently expressed the markers correlating with stemness.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1109/EMBC.2016.7591645 | DOI Listing |
Publication Analysis
Top Keywords
implants adipose
8
cells
5
bio-active polymer
4
polymer implants
4
adipose tissue
4
tissue situ
4
situ source
4
source reprogramming
4
reprogramming cells
4
cells implants
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!