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Myogenic Differentiation Potential of Mesenchymal Stem Cells Derived from Fetal Bovine Bone Marrow. | LitMetric

Myogenic Differentiation Potential of Mesenchymal Stem Cells Derived from Fetal Bovine Bone Marrow.

Anim Biotechnol

b Departamento de Fomento de la Producción Animal, Facultad de Ciencias Veterinarias y Pecuarias , Universidad de Chile , Santiago , Chile.

Published: January 2018

AI Article Synopsis

  • This study investigates the myogenic potential of bovine fetal mesenchymal stem cells (bfMSC) derived from bone marrow, which could improve livestock production through understanding muscle development.
  • Three differentiation protocols were tested using a DNA methyltransferase inhibitor (5-Aza), a myoblast-secreted factor (Galectin-1), and a specialized culture medium (SkGM-2 BulletKit).
  • Results showed that higher concentrations of 5-Aza enhanced muscle regulatory factor mRNA levels, indicating the protocols can effectively induce myogenic differentiation in bfMSC.

Article Abstract

The myogenic potential of bovine fetal MSC (bfMSC) derived from bone marrow (BM) remains unknown; despite its potential application for the study of myogenesis and its implications for livestock production. In the present study, three protocols for in vitro myogenic differentiation of bfMSC based on the use of DNA methyltransferase inhibitor 5-Aza-2'-deoxycytidine (5-Aza), myoblast-secreted factor Galectin-1 (Gal-1), and myoblast culture medium SkGM-2 BulletKit were used. Plastic-adherent bfMSC were isolated from fetal BM collected from abattoir-derived fetuses. Post-thaw viability analyses detected 85.6% bfMSC negative for propidium iodine (PI). Levels of muscle regulatory factors (MRF) MYF5, MYF6, MYOD, and DES mRNA were higher (P < 0.05) in bfMSC cultured under 100 µM of 5-Aza compared to 1 and 10 µM. Treatment of bfMSC with 10 µM of 5-Aza resulted in down-regulation of MYOD mRNA (Days 7 to 21) and up-regulation of MYF6 (Day 7), MYF5, and DES mRNA (Day 21). Gal-1 and SkGM-2 BulletKit induced sequential down-regulation of early MRF (MYF5) and up-regulation of intermediate (MYOD) and late MRF (DES) mRNA. Moreover, DES and MYF5 were immunodetected in differentiated bfMSC. In conclusion, protocols evaluated in bfMSC induced progress into myogenic differentiation until certain extent evidenced by changes in MRF gene expression.

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Source
http://dx.doi.org/10.1080/10495398.2016.1276926DOI Listing

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