Mass spectrometric identification of proteins in species lacking validated sequence information is a major problem in veterinary science. In the present study, we used ochratoxin A producing Penicillium verrucosum to identify and quantitatively analyze proteins of an organism with yet no protein information available. The work presented here aimed to provide a comprehensive protein identification of P. verrucosum using shotgun proteomics. We were able to identify 3631 proteins in an "ab initio" translated database from DNA sequences of P. verrucosum. Additionally, a sequential window acquisition of all theoretical fragment-ion spectra analysis was done to find differentially regulated proteins at two different time points of the growth curve. We compared the proteins at the beginning (day 3) and at the end of the log phase (day 12).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/pmic.201600467 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring a Cheese Ripening Process That Hinders Ochratoxin A Production by and .
Biology (Basel)
August 2024
Higiene y Seguridad Alimentaria, Instituto de Investigación de Carne y Productos Cárnicos (IProCar), Facultad de Veterinaria, Universidad de Extremadura, Avda. de las Ciencias s/n, 10003 Cáceres, Spain.
A lack of control of the technological abiotic parameters apparent during cheese manufacture, including temperature and relative humidity, results in this dairy product being prone to mold contamination. Sometimes, inoculant molds are used to obtain the characteristic sensory properties of this type of product. However, during the maturation process, some unwanted molds can colonize the ripening cheese and produce mycotoxins.
View Article and Find Full Text PDFEco-friendly lignin nanoparticles as antioxidant and antimicrobial material for enhanced textile production.
Sci Rep
July 2024
Biochemistry Department, Faculty of Agriculture, Cairo University, Giza, Egypt.
Natural polymers are bioactive compounds that are used in the treatment of several disorders. Natural lignin, an amorphous polymer, offers significant potential for use as a building block in the production of bio-renovation materials. This study used an alkaline solvent technique to extract lignin from two Egyptian cotton cultivar byproducts, Giza 86 and 90.
View Article and Find Full Text PDFThe effect of the interactions of water activity, and temperature on OTA, OTB, and OTα produced by Penicillium verrucosum in a mini silo of natural and inoculated wheat using CO production as fungal activity sentinel.
Food Chem
December 2024
Applied Mycology Group, Environment and AgriFood Theme, Cranfield University, College Rd. Wharley End, Bedford MK43 0AL, UK. Electronic address:
Ochratoxin A (OTA) is a nephrotoxin that contaminates grains in storage. Moisture and temperature sensors give delayed responses due to their slow kinetic movement within the silo. This study examines if CO production could predict OTA contamination and identify storage conditions exceeding the maximum limit (5 μg/kg).
View Article and Find Full Text PDFBioactives of the essential oil from the leaves of Cambess (Myrtaceae) on the effects of tobacco.
Front Pharmacol
June 2024
Graduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense, Umuarama, Brazil.
Introduction: Lung cancer is the most commonly diagnosed and the main cause of cancer death, usually related to cigarette smoking. Furthermore, the microbiota of people exposed to cigarette smoke can be modified, making it difficult to eliminate opportunistic microorganisms. The leaves of are a by-product of fruit production and, to date, there have been no studies addressing the antiproliferative, anti-inflammatory, and antimicrobial activities.
View Article and Find Full Text PDFProduction of Ochratoxin A and Citrinin and the Expression of Their Biosynthetic Genes from in Liquid Culture.
ACS Omega
May 2024
Metrology, National Research Council, 1200 Montreal Road, Ottawa, Ontario K1A 0R6, Canada.
is a fungal pathogen capable of producing two mycotoxins of concern, ochratoxin A (OTA) and citrinin (CIT). The production profile of these two mycotoxins is not well understood but could help mitigate co-contamination in the food supply. As such, the production of OTA and CIT from DAOMC 242724 was investigated under different growing conditions in liquid culture.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!