Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340209PMC
http://dx.doi.org/10.1016/j.bpj.2017.01.014DOI Listing

Publication Analysis

Top Keywords

fluorescent labeling
4
labeling preserving
4
preserving ocp
4
ocp photoactivity
4
photoactivity reveals
4
reveals reorganization
4
reorganization photocycle
4
fluorescent
1
preserving
1
ocp
1

Similar Publications

Extracellular vesicles (EVs) have garnered attention in research for their potential as biochemical transporters and immune modulators, crucial for regulating the host immune system. The present study was conducted to isolate and characterize EVs from Gram negative bacteria (EVs) and investigate their proteomic profile and immune responses. Isolation of EVs was carried out using ultracentrifugation method.

View Article and Find Full Text PDF

One-step antifouling coating of polystyrene using engineered polypeptides.

J Colloid Interface Sci

January 2025

Physical Chemistry and Soft Matter, Wageningen University & Research, Stippeneng 4 6708 WE Wageningen, The Netherlands. Electronic address:

Unwanted nonspecific adsorption caused by biomolecules influences the lifetime of biomedical devices and the sensing performance of biosensors. Previously, we have designed B-M-E triblock proteins that rapidly assemble on inorganic surfaces (gold and silica) and render those surfaces antifouling. The B-M-E triblock proteins have a surface-binding domain B, a multimerization domain M and an antifouling domain E.

View Article and Find Full Text PDF

True cancer stem cells exhibit relative degrees of dormancy and genomic stability.

Neoplasia

January 2025

Department of Pathology, Anatomy and Cell Biology and the Clinical and Translational Research Center of Excellence, Meharry Medical College, 1005 Dr. D.B. Todd Jr. Boulevard, Nashville, TN 37208, USA.

Background: Cancer stem cells in human tumors have been defined by stem cell markers, embryonal signaling pathways and characteristic biology, ie., namely the ability to repopulate the proliferating population. However, even if these properties can be demonstrated within a tumor cell subpopulation, it does not mean that they are truly hierarchical stem cells because they could have been derived from the proliferating population in a reversible manner.

View Article and Find Full Text PDF

DNA Origami Framework-Based Spatial Nanochip for Circular ssDNA Assembly and Data Storage.

Small

January 2025

Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.

A 3D DNA spatial chip (DSC) based on an icosahedral DNA origami framework is introduced to construct customized circular single-stranded DNA (c-ssDNA) for data storage. Within the confined space of the DSC, thirty addressable location sequences extending from the framework edges are available for designing circular paths and directing the assembly of a series of information oligonucleotides for efficient ligation. This strategy is verified by constructing c-ssDNAs from up to 15 fragments to encode two poems (800 and 860 nucleotides).

View Article and Find Full Text PDF

The pulmonary vasculature plays a pivotal role in the development and progress of chronic lung diseases. Due to limitations of conventional two-dimensional histological methods, the complexity and the detailed anatomy of the lung blood circulation might be overlooked. In this study, we demonstrate the practical use of optical serial block face imaging (SBFI), ex vivo microcomputed tomography (micro-CT), and nondestructive optical tomography for visualization and quantification of the pulmonary circulation's 3D architecture from macro- to micro-structural levels in murine lung samples.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!