AI Article Synopsis
- Plant cells react to environmental stresses by producing reactive oxygen species (ROS), a typical cellular response.
- Luminol-based bioassays are effective for detecting ROS levels in plant tissue, particularly when exposed to stimuli like phytohormones.
- The study focuses on optimizing a luminol-based protocol to measure changes in hydrogen peroxide (HO) levels in Arabidopsis leaf discs after exposure to cytokinins.
Article Abstract
Plant cells respond to stresses and exogenous environmental stimuli. Production of altered levels of reactive oxygen species (ROS) is one of a typical cellular responses against such stimuli. These responses are detectable through various techniques including luminol-based bioassays. Luminol-based bioassays provide an excellent opportunity for detection of ROS in plant leaf tissue when challenged with some exogenous stimuli like phytohormones, cytokinins, auxins, ABA, etc. The luminol-based protocol in point aims at measuring changes in the HO levels of Arabidopsis leaf discs when exposed to phytohormone cytokinins. However, utility of the luminol-based ROS determination protocol is quite wide. We specifically optimized this protocol to quantify the effect of cytokinins on ROS production by Arabidopsis leaves.
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| http://dx.doi.org/10.1007/978-1-4939-6831-2_13 | DOI Listing |
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