The immuno-PCR (iPCR) method combines advantages of enzyme-linked immunosorbent assay and polymerase chain reaction, which is used in iPCR as a method of "visualization" of antigen-antibody interaction. The use of iPCR provides classical PCR sensitivity to objects traditionally detected by ELISA. This method could be very sensitive and allow for detection of quantities of femtograms/ml order. However, iPCR is still not widely used. The aim of this review is to highlight the special features of the iPCR method and to show the main aspects of its development and application in recent years.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1134/S0006297916130113 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Article Synopsis
- Facets synovial cysts (FSCs) are benign growths from facet joints that can lead to nerve pain, and this study explored the effectiveness of CT-guided procedures for their treatment, comparing IPCR and DF methods.
- The retrospective review examined 90 FSC rupture attempts on 75 patients over 10 years, revealing that IPCR alone had a 70% success rate, but when combined with DF after IPCR failure, the success rate rose to 90.6%.
- The study concluded that having the option to use DF after IPCR failure significantly improves treatment outcomes, highlighting the advantages of using CT guidance for these procedures.
Covert airflow obstruction dominates the overt ones in interstitial lung disease: An appraisal.
Indian J Med Res
July 2024
Department of Airway Diseases, Institute of Pulmocare & Research (IPCR), Kolkata, India.
Background & objectives The co-presence of non-emphysematous airflow obstruction in interstitial Lung disease (ILD) is not elaborated. The present study aims the job with spirometry. Methods ILD affected individuals with or without airflow obstruction (FEV1/FVC<0.
View Article and Find Full Text PDFCan immuno-PCR (IPCR) transform bacterial disease diagnostics?
Expert Rev Mol Diagn
October 2024
Adult Infectious Disease, Department of Medicine, Imperial College London, London, UK.
Article Synopsis
- Around 15 million people die from infectious diseases each year, so it's super important to diagnose them quickly to help people get better and stop the spread.
- A new method called Immuno Polymerase Chain Reaction (IPCR) is being studied for diagnosing bacterial diseases, and it’s better than older methods.
- Experts say IPCR can help find diseases faster and more accurately, which is super important for treatment, but it still needs some improvements to make it quicker and easier to use, especially in places with fewer resources.
Diagnosis of abdominal tuberculosis: Detection of mycobacterial CFP-10 and HspX proteins by gold nanoparticle-PCR amplified immunoassay.
J Microbiol Methods
May 2024
Faculty of Allied Health Sciences, Shree Guru Gobind Singh Tricentenary University, Gurugram 122505, India; Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak 124001, India. Electronic address:
Attempts were made to improve the efficacy of PCR amplified immunoassay (I-PCR) for diagnosing abdominal TB cases by utilizing the gold nanoparticle (AuNP)-based I-PCR, where AuNPs were functionalized with detection antibodies/oligonucleotides that exhibited 84.3% sensitivity and 95.1% specificity.
View Article and Find Full Text PDFThe pursuit of precision oncology heavily relies on large-scale genomic and pharmacological data garnered from preclinical cancer model systems such as cell lines. While cell lines are instrumental in understanding the interplay between genomic programs and drug response, it well-established that they are not fully representative of patient tumors. Development of integrative methods that can systematically assess the commonalities between patient tumors and cell-lines can help bridge this gap.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!