While RNA editing by A-to-I deamination is a requisite for neuronal function in humans, it is under-investigated in single cells. Here we fill this gap by analyzing RNA editing profiles of single cells from the brain cortex of living human subjects. We show that RNA editing levels per cell are bimodally distributed and distinguish between major brain cell types, thus providing new insights into neuronal dynamics.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435858 | PMC |
| http://dx.doi.org/10.1261/rna.058271.116 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enhancing virus-mediated genome editing for cultivated tomato through low temperature.
Plant Cell Rep
January 2025
Department of Horticultural Science, Kyungpook National University, Daegu, 41566, Republic of Korea.
Viral vector-mediated gene editing is enhanced for cultivated tomato under low temperature conditions, enabling higher mutation rates, heritable, and virus-free gene editing for efficient breeding. The CRISPR/Cas system, a versatile gene-editing tool, has revolutionized plant breeding by enabling precise genetic modifications. The development of robust and efficient genome-editing tools for crops is crucial for their application in plant breeding.
View Article and Find Full Text PDFDe novo assembly of the mitochondrial genome of Glycyrrhiza glabra and identification of two types of homologous recombination configurations caused by repeat sequences.
BMC Genomics
January 2025
School of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
Background: Glycyrrhiza glabra, which is widely used in medicine and therapy, is known as the 'king of traditional Chinese medicine'. In this study, we successfully assembled and annotated the mitochondrial and chloroplast genomes of G. glabra via high-throughput sequencing technology, combining the advantages of short-read (Illumina) and long-read (Oxford Nanopore) sequencing.
View Article and Find Full Text PDFComparative analysis of mitochondrial genomes of Stemona tuberosa lour. reveals heterogeneity in structure, synteny, intercellular gene transfer, and RNA editing.
BMC Plant Biol
January 2025
Dazhou Academy of Agricultural Sciences, Dazhou, 635000, China.
Background: Stemona tuberosa, a vital species in traditional Chinese medicine, has been extensively cultivated and utilized within its natural distribution over the past decades. While the chloroplast genome of S. tuberosa has been characterized, its mitochondrial genome (mitogenome) remains unexplored.
View Article and Find Full Text PDFCTGCT, Centre of Excellence for the Technologies of Gene and Cell Therapy: Collaborative translation of scientific discoveries into advanced treatments for neurological rare genetic diseases and cancer.
Comput Struct Biotechnol J
December 2024
Centre for the Technologies of Gene and Cell Therapy, National Institute of Chemistry, Hajdrihova 19, SI1000 Ljubljana, Slovenia.
The emerging field of precision medicine relies on scientific breakthroughs to understand disease mechanisms and develop cutting-edge technologies to overcome underlying genetic and functional aberrations. The establishment of the Centre of Excellence for the Technologies of Gene and Cell Therapy (CTGCT) at the National Institute of Chemistry (NIC) in Ljubljana represents a significant step forward, as it is the first centre of its kind in Slovenia. The CTGCT is poised to spearhead advances in cancer immunotherapy and personalised therapies for neurological and other rare genetic diseases.
View Article and Find Full Text PDFPrecise template-free correction restores gene function in Tay-Sachs disease while reframing is ineffective.
Mol Ther Nucleic Acids
March 2025
Genetics and Genome Biology Program, The Hospital for Sick Children, Toronto, ON M5G0A4, Canada.
Tay-Sachs disease is a fatal neurodegenerative disorder caused by mutations inactivating the metabolic enzyme HexA. The most common mutation is c.1278insTATC, a tandem 4-bp duplication disrupting expression by frameshift.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!