Analyzing the Dynamics of Signaling Microclusters.

Methods Mol Biol

Laboratory for Cell Signaling, RIKEN Center for Integrative Medical Sciences., 1 7 22 Suehiro-cho, Yokohama, 230 0045, Japan.

Published: February 2018

T cell antigen receptor (TCR) stimulation induces recruitment and accumulation of various types of signaling molecules and forms signaling microclusters. The dynamics of the microclusters are important for regulating the quality and quantity of T cell activation. We describe here our protocols for analysis of signaling microclusters by using supported planar bilayers.

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http://dx.doi.org/10.1007/978-1-4939-6881-7_4DOI Listing

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