AI Article Synopsis

  • Spinal cord injury (SCI) currently has no effective treatment, but a study tested a composite treatment system using a silk fibroin/alginates scaffold combined with neurotrophic factors and stem cells in rats.
  • The silk fibroin scaffold serves as a biomimetic platform, promoting neuron accommodation and tissue rebuilding, with the addition of GDNF improving neuron survival and spinal cord recovery.
  • The results suggest that this composite approach could enhance therapeutic strategies for SCI, laying groundwork for potential clinical applications.

Article Abstract

Spinal cord injury (SCI) is a severe trauma for which no effective treatment is currently available. In this study, a composited treatment system was prepared using a silk fibroin/alginates/glial cell line-derived neurotrophic factor (SF/AGs/GDNF) scaffold seeded with human umbilical cord mesenchymal stem cells (hUCMSCs) and the combined therapeutic effects of the composite scaffold to repair SCI rats were evaluated. The use of SF as a scaffold material could act as a biomimetic platform allowing neurons to properly accommodate and rebuild the target tissue. The SF/AGs/GDNF scaffold had the best sustained-release function and the AGs were the key determining factor in the controlled release of GDNF. After 8weeks of treatment, the hUCMSCs on SF/AGs/GDNF composite scaffolds could significantly enhance the scar expansion of spinal cord tissue and increased the number of surviving neurons. The combination of GDNF and hUCMSCs transplantation loaded on SF/AGs composite scaffolds exhibited better therapeutic and repair effects to the SCI of rats, compared with the SF/AGs group or GDNF alone on SF/AGs scaffolds. The composite scaffold, GDNF and stem cells could build a bioactive material to form the micro-environment of growth and repair of the neurons. These results may provide a theoretical basis and beneficial exploration for clinical treatment of SCI.

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Source
http://dx.doi.org/10.1016/j.msec.2016.12.017DOI Listing

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