AI Article Synopsis
- A new sample preservation method for single-cell RNA preparation allows for the maintenance of viable cells and their transcripts, overcoming limitations of current protocols that require fresh material.
- This method enables researchers to dissociate the timing and location of cell sampling from later processing, facilitating more complex experimental designs.
- Sequencing results from both fresh and cryopreserved cells show that the preservation process does not impact transcriptional profiles, potentially transforming the field of single-cell transcriptomics.
Article Abstract
A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333448 | PMC |
| http://dx.doi.org/10.1186/s13059-017-1171-9 | DOI Listing |
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