AI Article Synopsis
- Probing protein kinases in living cells is challenging and demands specialized imaging tools.
- The rise of genetically encoded and synthetic fluorescent biosensors allows for real-time monitoring of these kinases in their natural environment without damaging the cells.
- The review discusses current technologies, highlights their limitations, and calls for advancements, especially in infrared probes and methods to enhance signal clarity and controlled probe activation.
Article Abstract
Probing the dynamic activities of protein kinases in real-time in living cells constitutes a major challenge that requires specific and sensitive tools tailored to meet the particular demands associated with cellular imaging. The development of genetically-encoded and synthetic fluorescent biosensors has provided means of monitoring protein kinase activities in a non-invasive fashion in their native cellular environment with high spatial and temporal resolution. Here, we review existing technologies to probe different dynamic features of protein kinases and discuss limitations where new developments are required to implement more performant tools, in particular with respect to infrared and near-infrared fluorescent probes and strategies which enable improved signal-to-noise ratio and controlled activation of probes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5217393 | PMC |
| http://dx.doi.org/10.3390/proteomes3040369 | DOI Listing |
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