The Pollen Coat Proteome: At the Cutting Edge of Plant Reproduction.

Proteomes

Plant Reproductive Biology Laboratory, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008 Granada, Spain.

Published: January 2016

The tapetum is a single layer of secretory cells which encloses the anther locule and sustains pollen development and maturation. Upon apoptosis, the remnants of the tapetal cells, consisting mostly of lipids and proteins, fill the pits of the sculpted exine to form the bulk of the pollen coat. This extracellular matrix forms an impermeable barrier that protects the male gametophyte from water loss and UV light. It also aids pollen adhesion and hydration and retains small signaling compounds involved in pollen-stigma communication. In this study, we have updated the list of the pollen coat's protein components and also discussed their functions in the context of sexual reproduction.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5217362PMC
http://dx.doi.org/10.3390/proteomes4010005DOI Listing

Publication Analysis

Top Keywords

pollen coat
8
pollen
5
coat proteome
4
proteome cutting
4
cutting edge
4
edge plant
4
plant reproduction
4
reproduction tapetum
4
tapetum single
4
single layer
4

Similar Publications

PCP-B peptides and CrRLK1L receptor kinases control pollination via pH gating of aquaporins in Arabidopsis.

Dev Cell

January 2025

School of Life Sciences, East China Normal University, Shanghai 200241, China; Institute of Eco-Chongming, East China Normal University, Shanghai 202162, China. Electronic address:

During pollen-stigma interaction, pollen coat protein B-class peptides (PCP-Bs) compete with stigmatic rapid alkalinization factor (RALF) for interaction with FERONIA/ANJEA receptor kinases (FER/ANJ), stimulating pollen hydration and germination. However, the molecular mechanism underlying PCP-Bs-induced, FER/ANJ-regulated compatible responses remains largely unknown. Through PCP-Bγ-induced phosphoproteomic analysis, we characterized a series of pollination-related signaling pathways regulated by FER/ANJ.

View Article and Find Full Text PDF
Article Synopsis
  • Melilotus sulcatus is a hardy annual weed found in the Mediterranean and Asia, known for its durable seed coat that enhances seed dormancy and longevity in the seed bank.
  • The study examined the effects of different temperatures on seed germination and explored the phenology and reproductive biology of various populations from two distinct climate regions in Israel.
  • Results revealed variations in seed characteristics and optimal germination temperatures between populations, with field populations producing heavier seeds, but no significant growth differences in final weight or height between the two locations.
View Article and Find Full Text PDF

Hybridization barriers in Brassicaceae play a pivotal role in governing reproductive success and maintaining speciation. In this perspective, we highlight recent advances revealing the intricate molecular mechanisms and the interplay among key players governing these barriers. Recent studies have shed light on the molecular mechanisms that govern hybridization barriers in Brassicaceae.

View Article and Find Full Text PDF

A protocol for in vivo RNA labeling and visualization in tobacco pollen tubes.

STAR Protoc

December 2024

Laboratory of Pollen Biology, Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 00 Prague, Czech Republic. Electronic address:

Here, we present a protocol for labeling and live visualization of RNA-protein complexes in the form of ribonucleoprotein particles (RNPs) in tobacco pollen tubes. We describe steps for constructing RNA-pp7/MS2 tag and biolistic gene-gun-mediated pollen transformation. We then provide detailed procedures for RNA labeling using PP7 aptamer nascent RNA tagging and a fluorescently labeled Pseudomonas aeruginosa PP7 bacteriophage coat protein (PCP) reporter that binds to PP7 RNA stem loops.

View Article and Find Full Text PDF

Genome-wide identification, transcript profiling and functional analyses of PCP gene family in Wucai (Brassica campestris).

Sci Rep

November 2024

Anhui Provincial Engineering Center of Horticultural Crop Breeding, College of Horticulture, Anhui Agricultural University, Hefei, 230036, China.

Article Synopsis
  • Pollen coat proteins (PCPs) are small protein molecules crucial for pollen functions like wall synthesis and germination, but their role in Wucai (Brassica campestris L.) is still unclear.
  • A study identified 20 BcPCP genes, categorized into three subfamilies, indicating that these genes are more active in reproductive organs and likely important for reproductive development.
  • The study highlighted BcGRP20, a gene related to male sterility, showing that its overexpression can restore fertility in an Arabidopsis mutant, suggesting its essential role in pollination and potential for understanding male infertility in Wucai.
View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!