AI Article Synopsis
- The study aimed to assess how RepSox, a small molecule, impacts gene expression and the development of embryos created through interspecies somatic cell nuclear transfer (iSCNT) involving rhesus monkeys and pigs.
- Results showed that RepSox increased the levels of certain important genes related to cell growth and survival during the early stages of embryo development, although its effect on blastocyst developmental rates was not statistically significant.
- The findings suggest that RepSox enhances the potential for development in these iSCNT embryos by fine-tuning the expression of key pluripotency genes.
Article Abstract
Objective: To investigate the effect of the small molecule, RepSox, on the expression of developmentally important genes and the pre-implantation development of rhesus monkey-pig interspecies somatic cell nuclear transfer (iSCNT) embryos.
Results: Rhesus monkey cells expressing the monomeric red fluorescent protein 1 which have a normal (42) chromosome complement, were used as donor cells to generate iSCNT embryos. RepSox increased the expression levels of the pluripotency-related genes, Oct4 and Nanog (p < 0.05), but not of Sox2 compared with untreated embryos at the 2-4-cell stage. Expression of the anti-apoptotic gene, Bcl2, and the pro-apoptotic gene Bax was also affected at the 2-4-cell stage. RepSox treatment also increased the immunostaining intensity of Oct4 at the blastocyst stage (p < 0.05). Although the blastocyst developmental rate was higher in the group treated with 25 µM RepSox for 24 h than in the untreated control group (2.4 vs. 1.2%, p > 0.05), this was not significant.
Conclusion: RepSox can improve the developmental potential of rhesus monkey-pig iSCNT embryos by regulating the expression of pluripotency-related genes.
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| http://dx.doi.org/10.1007/s10529-017-2308-3 | DOI Listing |
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