Placentas from women aged 25-32 years with normal course of gestation were studied. It is essential to stick to certain methodological approaches for preparing viable multipotent mesenchymal stromal cell culture and to carry out morphological (macro and micro) evaluation of the chorionic villi, umbilical cords, and placentas. At stage I of the study, patients' histories, labor course, and examinations of the newborns should be analyzed to exclude women with genital and extragenital diseases. At stage II, it is essential to stick to special regulations and methods for collection of specimens of the cord, amnion, and placental tissue proper. Histological control of the placental structures collected for multipotent mesenchymal stromal cell culturing is obligatory.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10517-017-3648-7DOI Listing

Publication Analysis

Top Keywords

multipotent mesenchymal
12
mesenchymal stromal
12
placental tissue
8
essential stick
8
stromal cell
8
conditions collection
4
collection placental
4
tissue samples
4
samples culturing
4
culturing multipotent
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!