Nucleotides were effective in inhibiting the class C β-lactamase CMY-10. IMP was the most potent competitive inhibitor, with a value of 16.2 μM. The crystal structure of CMY-10 complexed with GMP or IMP revealed that nucleotides fit into the R2 subsite of the active site with a unique vertical binding mode where the phosphate group at one terminus is deeply bound in the subsite and the base at the other terminus faces the solvent.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404520 | PMC |
| http://dx.doi.org/10.1128/AAC.00098-17 | DOI Listing |
Publication Analysis
Top Keywords
gmp imp
8
class β-lactamase
8
imp competitive
4
competitive inhibitors
4
inhibitors cmy-10
4
cmy-10 extended-spectrum
4
extended-spectrum class
4
β-lactamase nucleotides
4
nucleotides effective
4
effective inhibiting
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!