AI Article Synopsis
- The portal vertex of dsDNA bacteriophage is crucial for both packaging and releasing the viral genome.
- The termination of DNA packaging is influenced by the density of the DNA already packaged, with the portal protein acting as a sensor that responds to this density through changes in its structure.
- This study investigates how specific mutations in the portal core affect DNA packaging density in P22, revealing that certain structural changes can significantly increase the amount of DNA packaged, suggesting the importance of portal core compression in the packaging process.
Article Abstract
The portal vertex in dsDNA bacteriophage serves as the site for genome encapsidation and release. In several of these viruses, efficient termination of DNA packaging has been shown to be dependent on the density of packaged DNA. The portal protein has been implicated as being part of the sensor that regulates packaging termination through DNA-dependent conformational changes during packaging. The mechanism by which DNA induces these conformational changes remains unknown. In this study, we explore how point mutants in the portal core can result in changes in genome packaging density in P22. Mutations in the portal core that subtly alter the structure or dynamics of the protein result in an increase in the amount of DNA packaged. The magnitude of the change is amino acid and location specific. Our findings suggest a mechanism wherein compression of the portal core is an essential aspect of signal transmission during packaging.
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| http://dx.doi.org/10.1016/j.virol.2017.02.019 | DOI Listing |
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