The mechanisms of associative long-lasting potentiation (LLP) of excitatory postsynaptic potentials (EPSPs) were studied in the motor cortex of anesthetized cats. Mono- and oligosynaptic EPSPs were evoked by stimulations of thalamic VL nucleus, pyramidal tract, callosal and somatosensory system and paired with orthodromic, antidromic or current-induced action potentials. EPSP-spike stimulus pairs with 0.1-0.2 Hz frequency and 0-200 ms interstimulus intervals induced increases in the amplitudes and durations of EPSPs for 40-60 min or longer after 20-50 pairings. The LLP was prevented when postsynaptic firing was blocked by intracellular current injection or by juxtasomatic application of gamma-aminobutyric acid. LLP was also prevented when the level of intracellular free calcium was lowered by the intracellular injection of the calcium chelator EGTA or when neuronal transport was blocked by the intracellular injection of colchicine. Neither EGTA nor colchicine blocked postsynaptic firing. Thus, these findings show that LLP in the neocortex is a postsynaptic phenomenon which requires conjunctive pre- and postsynaptic activity, adequate levels of intracellular free calcium, and functional intracellular transport.
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J Colloid Interface Sci
April 2025
High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Science, Hefei, Anhui 230031, PR China; University of Science and Technology of China, Hefei, Anhui 230026, PR China. Electronic address:
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View Article and Find Full Text PDFInt J Biol Macromol
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Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China. Electronic address:
TEMPO-CNF film modified by two-dimension transition metal MXene has certain antibacterial properties. However, the problem of long-lasting stability greatly restricts the feasibility of long-term use of the composite film. Here, we introduced polyaniline (PANI) as a modifying molecule, which was electrostatically adsorbed on the surface of the MXene nanosheets to prevent its self-stacking and delay its oxidation.
View Article and Find Full Text PDFViruses
January 2025
Department of Biochemistry and Molecular Biotechnology, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
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View Article and Find Full Text PDFPharmaceuticals (Basel)
December 2024
Faculty of Chemical Engineering and Biotechnology, National University of Science and Technology POLITEHNICA of Bucharest, Polizu No 1, 011061 Bucharest, Romania.
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View Article and Find Full Text PDFPathogens
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Department of Biomedical Sciences, Parasitology Division, Faculty of Medicine, Universitas Padjadjaran, Bandung 45363, Indonesia.
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