Zonal sedimentation analysis on sucrose gradients allows estimation of the molecular size of an individual protein or a protein complex by centrifugation at a constant speed under nondenaturing conditions. This method is particularly suitable for globular proteins like the influenza A virus (IAV) protein hemagglutinin (HA). Here, I describe step by step a protocol used to evaluate the oligomeric state of recombinant HA trimers (Magadan , 2013).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321548 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.1100 | DOI Listing |
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