Acyltransferases are key contributors to triacylglycerol (TAG) synthesis and, thus, are of great importance for seed oil quality. The effects of increased or decreased expression of () or () on seed lipid composition were assessed in several lines. Furthermore, in vitro assays of acyltransferases in microsomal fractions prepared from developing seeds of some of these lines were performed. Decreased expression of led to an increased percentage of 18:3-3 without any change in total lipid content of the seed. The tri-18:3 TAG increase occurred predominantly in the cotyledon, as determined with matrix-assisted laser desorption/ionization-mass spectrometry, whereas species with two 18:3-3 acyl groups were elevated in both cotyledon and embryonal axis. overexpression led to a relative increase of 18:2-6 at the expense of 18:3-3, also without affecting the total lipid content. Differential distributions of TAG species also were observed in different parts of the seed. The microsomal assays revealed that seeds have very high activity of diacylglycerol-phosphatidylcholine interconversion. The combination of analytical and biochemical data suggests that the higher 18:2-6 content in the seed oil of the overexpressors is due to the channeling of fatty acids from phosphatidylcholine into TAG before being desaturated to 18:3-3, caused by the high activity of PDAT in general and by PDAT specificity for 18:2-6. The higher levels of 18:3-3 in -silencing lines are likely due to the compensatory activity of a TAG-synthesizing enzyme with specificity for this acyl group and more desaturation of acyl groups occurring on phosphatidylcholine.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373062 | PMC |
http://dx.doi.org/10.1104/pp.16.01865 | DOI Listing |
Sci Adv
January 2025
College of Life Science and Technology, Key Laboratory of Molecular Biophysics of the Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China.
Yellow seed coat color (SCC) is a valuable trait in , which is significantly correlated to high seed oil content (SOC) and low seed lignocellulose content (SLC). However, no dominant yellow SCC genes were identified in . In this study, a dominant yellow SCC N53-2 was verified, and then 58,981 eQTLs and 25 trans-eQTL hotspots were identified in a double haploid population derived from N53-2 and black SCC material Ken-C8.
View Article and Find Full Text PDFFood Sci Technol Int
January 2025
Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, Maslak, Istanbul, Türkiye.
This study aimed to evaluate the antimicrobial effectiveness of cumin seed essential oil (CEO) after encapsulation in chickpea protein-maltodextrin matrix by spray drying and to provide insight into potential use as a natural ingredient in meat-based products. The surface morphology results of encapsulated CEO showed the dispersion in the wall material matrix, and the observed specific common peaks in the FT-IR spectra of encapsulated and non-encapsulated CEO proved the successful encapsulation. The antibacterial activity of non-encapsulated CEO against BC1402, ATCC 27853, Typhimurium ATCC 0402, ATCC 25923 were first evaluated by disc diffusion assay.
View Article and Find Full Text PDFComplement Ther Med
December 2024
Department of Operating Room Nursing, Abadan University of Medical Sciences, Abadan, Iran. Electronic address:
Objectives: Recent randomized controlled trials (RCTs) have studied the potential effect of the topical use of sesame oil (SO), obtained from the sesame plant seeds (Sesamum indicum L., Pedaliaceae family), in preventing or alleviating the symptoms of infusion-related phlebitis (IRP); nevertheless, their data are inconsistent. Thus, this review sought to qualitatively and quantitatively synthesize data from all available RCTs concerning the effect of the topical administration of SO on managing IRP.
View Article and Find Full Text PDFFood Chem
December 2024
College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China. Electronic address:
The demand for sustainable plant-based protein is rising due to concerns over the environmental impact of animal-based protein. One promising yet overlooked protein source is the seed cake generated from Camellia oleifera oil extraction (COSC), which contains 14-20 % crude protein. COSC protein (COSCP) exhibit excellent nutritional and functional properties making it a promising ingredient for innovative food products.
View Article and Find Full Text PDFSci Rep
December 2024
Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS), P.O. Box 6091, Damascus, Syria.
Paclitaxel, a powerful anticancer drug, is limited by its poor water solubility and systemic toxicity, which hinder its effectiveness against aggressive brain tumors. This study aims to overcome these challenges by exploring novel intranasal delivery methods using lipid droplets (LDs) derived from date palm seeds (DPLDs) and mouse liver (MLLDs). The anticancer efficacy of PTX was evaluated using a comparative intranasal delivery approach.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!