To develop a sensitive fluorescence polarization immunoassay (FPIA) for screening the zearalenone class of mycotoxins in maize, two new monoclonal antibodies with uniform affinity to the zearalenone class and four fluorescein-labeled tracers were prepared. After careful selection of appropriate tracer-antibody pairs in terms of sensitivity and specificity, a FPIA that could simultaneously detect the zearalenone class with similar sensitivity was developed. Under optimum conditions, the half maximal inhibitory concentrations of the FPIA in buffer were 1.89, 1.97, 2.43, 1.99, 2.27, and 2.44 μg/L for zearalenone, α-zearalenol, β-zearalenol, α-zearalanol, β-zearalanol, and zearalanone, respectively. The limit of detection of FPIA for the zearalenone class was around 12 μg/kg in maize, and the recoveries ranged from 84.6 to 113.8%, with coefficients of variation below 15.3% in spiked samples. Finally, the FPIA was applied for screening naturally contaminated maize samples, and the results indicated a good correlation with that of high-performance liquid chromatography-tandem mass spectrometry.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.jafc.6b05614 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Review: Strategies and technologies in preventing regulated and emerging mycotoxin co-contamination in forage for safeguarding ruminant health.
Animal
September 2024
Department of Animal Science, Food and Nutrition DIANA, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, 29100 Piacenza, Italy.
Ruminants are often considered less susceptible to mycotoxins than monogastrics, owing to rumen microflora converting mycotoxins to less toxic compounds or several compounds present in the rumen-reticulum compartment, being able to bind the mycotoxin "mother" molecule that make them unavailable for absorption process in the gastro-intestinal tract of host animals. However, if ruminants consume feed contaminated by mycotoxins for long periods, their growth, development, and fertility can be compromised. Among regulated mycotoxins, the most studied and known for their effects are aflatoxins (AFs) AFB1, AFB2, AFG1 and AFG2, as well as the AFM1 for its high importance in dairy sector, deoxynivalenol (DON) and its metabolites 3/15 acetyl-DON and 3-glucoside DON, T-2 and HT-2 toxins, zearalenone, fumonisins, in particular that belong to the B class, and ochratoxin A.
View Article and Find Full Text PDFFumonisins alone or mixed with other fusariotoxins increase the C22-24:C16 sphingolipid ratios in chicken livers, while deoxynivalenol and zearalenone have no effect.
Chem Biol Interact
May 2024
ITAVI, L'Orfrasière, 37380, NOUZILLY, France. Electronic address:
Poultry feed is often contaminated with fumonisins, deoxynivalenol, and zearalenone, which can result in oxidative damage, inflammation and change in lipid metabolism. Although sphingolipids play key roles in cells, only the effects of fumonisins on the sphingolipidome are well-documented. In chickens, fumonisins have been shown to increase the sphinganine to sphingosine ratio and the C22-24:C16 sphingolipid ratio, which has been proposed as a new biomarker of toxicity.
View Article and Find Full Text PDFRole of ferroptosis in food-borne mycotoxin-induced toxicities.
Apoptosis
April 2024
College of Food Science and Nutritional Engineering, Beijing Key Laboratory for Food Non-thermal Processing, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing, 100083, China.
Contamination by toxic substances is a major global food safety issue, which poses a serious threat to human health. Mycotoxins are major class of food contaminants, mainly including aflatoxins (AFs), zearalenone (ZON), deoxynivalenol (DON), ochratoxin A (OTA), fumonisins (FBs) and patulin (PAT). Ferroptosis is a newly identified iron-dependent form of programmed or regulated cell death, which has been found to be involved in diverse pathological conditions.
View Article and Find Full Text PDFLncRNA profiles of Cyanidin-3-O-glucoside ameliorated Zearalenone-induced damage in porcine granulosa cells.
Gene
October 2023
College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China. Electronic address:
Long non-coding RNA (lncRNA), a class of RNA molecules with transcripts longer than 200 nt, is crucial for maintaining animal reproductive function. Zearalenone (ZEN) damaged animal reproduction by targeting ovarian granulosa cells (GCs), especially in pigs. Nonetheless, it is not quite clear that whether Cyanidin-3-O-glucoside (C3G) exert effects on porcine GCs (pGCs) after ZEN exposure by altering lncRNA expression.
View Article and Find Full Text PDFMetabolomic Analysis of Extracellular Vesicles from the Cereal Fungal Pathogen .
J Fungi (Basel)
April 2023
Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Bundoora 3086, Australia.
() is a filamentous fungus that infects cereals such as corn, wheat, and barley, with serious impact on yield as well as quality when the grain is contaminated with mycotoxins. Despite the huge impact of on food security and mammalian health, the mechanisms used by to export virulence factors during infection are not fully understood and may involve non-classical secretory pathways. Extracellular vesicles (EVs) are lipid-bound compartments produced by cells of all kingdoms that transport several classes of macromolecules and are implicated in cell-cell communication.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!