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Determination of glucose exchange rates and permeability of erythrocyte membrane in preeclampsia and subsequent oxidative stress-related protein damage using dynamic-F-NMR. | LitMetric

AI Article Synopsis

  • The study investigates the link between oxidative stress, endothelial dysfunction, and preeclampsia (PE) by examining the effect of a specific metabolite on red blood cell membranes.
  • Researchers used Dynamic-NMR to analyze how a fluorinated glucose mimic, 3-FDG, crosses the membranes of erythrocytes from both healthy pregnant women and those with PE.
  • The results showed no significant differences in the rate of 3-FDG exchange or membrane permeability between the two groups, suggesting that oxidative damage to the cell membranes is not present at the studied site.

Article Abstract

The cause of the pregnancy condition preeclampsia (PE) is thought to be endothelial dysfunction caused by oxidative stress. As abnormal glucose tolerance has also been associated with PE, we use a fluorinated-mimic of this metabolite to establish whether any oxidative damage to lipids and proteins in the erythrocyte membrane has increased cell membrane permeability. Data were acquired using F Dynamic-NMR (DNMR) to measure exchange of 3-fluoro-3-deoxyglucose (3-FDG) across the membrane of erythrocytes from 10 pregnant women (5 healthy control women, and 5 from women suffering from PE). Magnetisation transfer was measured using the 1D selective inversion and 2D EXSY pulse sequences, over a range of time delays. Integrated intensities from these experiments were used in matrix diagonalisation to estimate the values of the rate constants of exchange and membrane permeability. No significant differences were observed for the rate of exchange of 3-FDG and membrane permeability between healthy pregnant women and those suffering from PE, leading us to conclude that no oxidative damage had occurred at this carrier-protein site in the membrane.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346149PMC
http://dx.doi.org/10.1007/s10858-017-0092-yDOI Listing

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