Characterization of microsatellite markers in two exploited African trees, and (Meliaceae).

Appl Plant Sci

Evolutionary Biology and Ecology Unit, CP 160/12, Faculté des Sciences, Université Libre de Bruxelles, Av. F. D. Roosevelt 50, B-1050 Brussels, Belgium.

Published: February 2017

Premise Of The Study: Multiplexes of nuclear microsatellite primers were developed to investigate population genetic structure and diversity in two exploited African rainforest trees: and (Meliaceae).

Methods And Results: Microsatellite isolation was performed simultaneously on two nonenriched genomic libraries after next-generation sequencing. We developed 16 and 22 polymorphic markers for and in three and four multiplexes, respectively. The number of alleles ranged from two to 17 for and from three to 19 for . Mean expected and observed heterozygosity ranged between 0.75 ± 0.13 and 0.55 ± 0.23 for and between 0.73 ± 0.10 and 0.49 ± 0.2 for .

Conclusions: These sets of nuclear microsatellite markers constitute useful tools for exploring gene flow patterns in these two species.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5315381PMC
http://dx.doi.org/10.3732/apps.1600130DOI Listing

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