In modern resistance breeding, effectors have emerged as tools for accelerating and improving the identification of immune receptors. Effector-assisted breeding was pioneered for identifying resistance genes (R genes) against Phytophthora infestans in potato (Solanum tuberosum). Here we show that effectoromics approaches are also well suitable for identifying pathogen recognition receptors (PRRs) that recognize apoplastic effectors. To detect genotypes that recognize apoplastic proteins of P. infestans, routine agroinfiltration and potato virus X (PVX) agroinfection methods can be applied. In addition, protein infiltrations are feasible for assessing responses to apoplastic effectors and aid in confirming results obtained from the aforementioned methods. Protocols for the effectoromics pipeline are provided, starting from phenotyping for effector responses, up to genotyping and PRR gene identification.
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Article Synopsis
- Recognizing pathogen-associated molecular patterns is essential for plant immunity, and secreted proteases like SBT5.2a play a role in the stability of these patterns.
- The study shows that SBT5.2a cleaves the immunogenic epitope csp22 from cold-shock proteins (CSPs) of *Pseudomonas syringae*, reducing the plant's immune response.
- Plants lacking SBT5.2a have higher levels of csp22, leading to stronger immune responses and less pathogen growth, indicating that bacterial variations in csp22 stability affect plant-bacteria interactions.
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