Diagnosis and treatment of circadian rhythm sleep-wake disorders both require assessment of circadian phase of the brain's circadian pacemaker. The gold-standard univariate method is based on collection of a 24-hr time series of plasma melatonin, a suprachiasmatic nucleus-driven pineal hormone. We developed and validated a multivariate whole-blood mRNA-based predictor of melatonin phase which requires few samples. Transcriptome data were collected under normal, sleep-deprivation and abnormal sleep-timing conditions to assess robustness of the predictor. Partial least square regression (PLSR), applied to the transcriptome, identified a set of 100 biomarkers primarily related to glucocorticoid signaling and immune function. Validation showed that PLSR-based predictors outperform published blood-derived circadian phase predictors. When given one sample as input, the R of predicted vs observed phase was 0.74, whereas for two samples taken 12 hr apart, R was 0.90. This blood transcriptome-based model enables assessment of circadian phase from a few samples.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5318160 | PMC |
| http://dx.doi.org/10.7554/eLife.20214 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Diurnal Variation in Mitochondrial Gene in Human Type 2 Diabetic Mesenchymal Stem Cell Grafts.
Int J Mol Sci
January 2025
Division of Pharmaceutics, Faculty of Pharmaceutical Sciences, Sanyo-Onoda City University, Yamaguchi 756-0884, Japan.
The application of regenerative therapy through stem cell transplantation has emerged as a promising avenue for the treatment of diabetes mellitus (DM). Transplanted tissue homeostasis is affected by disturbances in the clock genes of stem cells. The aim of this study is to investigate the diurnal variation in mitochondrial genes and function after transplantation of adipose-derived mesenchymal stem cells (T2DM-ADSCs) from type 2 diabetic patients into immunodeficient mice.
View Article and Find Full Text PDFMaternal, Paternal, and Dual-Parental Alcohol Exposures Result in Both Overlapping and Distinct Impacts on Behavior in Adolescent Offspring.
Alcohol
January 2025
Department of Veterinary Physiology & Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA, 77843. Electronic address:
Emerging research reveals that alcohol use by fathers before conception can affect the growth and development of their offspring. Here, we used a C57BL/6J mouse model to study the effects of alcohol exposure on the behavior of the first-generation (F1) offspring, comparing the impacts of alcohol exposure by mothers, fathers, and both parents. Our goal was to determine how alcohol exposure by each parent or both parents influences the behavior of the offspring.
View Article and Find Full Text PDFGeneral procrastination associated with the evening preference in healthy people but not with circadian rhythm sleep-wake disorders with phase delay.
Chronobiol Int
January 2025
Department of Psychiatry, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Procrastination behavior has been reportedly associated with the evening preference. This study aimed to evaluate its difference between patients with circadian rhythm sleep-wake disorders with phase delay (CRSWDswPD) and healthy controls in terms of evening preference and comorbid psychiatric disorders. Thirty patients with CRSWDswPD and 29 healthy participants were included.
View Article and Find Full Text PDFModeling of Jet Lag and Searching for an Optimal Light Treatment.
J Biol Rhythms
January 2025
Department of Physics and i3n, University of Aveiro, Aveiro, Portugal.
The role of the hierarchical organization of the suprachiasmatic nucleus (SCN) in its functioning, jet lag, and the light treatment of jet lag remains poorly understood. Using the core-shell model, we mimic collective behavior of the core and shell populations of the SCN oscillators in transient states after rapid traveling east and west. The existence of a special region of slow dynamical states of the SCN oscillators can explain phenomena such as the east-west asymmetry of jet lag, instances when entrainment to an advance is via delay shifts, and the dynamics of jet lag recovery time.
View Article and Find Full Text PDFBimodal Patterns of Locomotor Activity and Sleep in : A Model for Their Simulation.
Nat Sci Sleep
January 2025
Department of Insect Genetics, Institute of Cytology and Genetics of the Siberian Branch, the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
Purpose: Two previously proposed modelling approaches to explain the bimodal pattern of activity and/or sleep in are based on 1) the concept of morning and evening oscillators underlying the peaks of activity in the morning and evening, respectively, and 2) the concept of two cycles of buildup and decay of sleep pressure, gated only by the circadian oscillator. Previously, we simulated 24-h alertness-sleepiness curves in humans using a model postulating the circadian modulation of the buildup and decay phases of two (wake and sleep) homeostatic processes. Here, we tested whether a similar model could be applied to simulate the bimodal 24-h rhythm of fly locomotor activity and sleep.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!