Herein we demonstrate a systematic investigation of chemically functionalizable, non-biofouling agarose films over large-area glass surfaces. Agarose films, prepared with various concentrations of aqueous agarose, were activated by using periodate oxidation to generate aldehyde groups at the termini of the agarose chains. The non-biofouling efficacy and binding capabilities of the activated films were evaluated by using protein and cellular patterning, performed by using a microarrayer, microcontact printing, and micromolding in capillaries. Characterization by using a fluorescence slide scanner and a scanning-probe microscope revealed that the pore sizes of the agarose films played an important role in achieving desirable film performance; the 0.2 wt % agarose film exhibited the optimum efficacy in this work.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/asia.201700010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Synergistic optical sensing of heavy metal ions using Mesua Ferrea derived carbon dot embedded Alginate based biopolymeric film as a sensor platform.
Int J Biol Macromol
December 2024
Chemical Engineering Group and Centre for Petroleum Research, CSIR-North East Institute of Science and Technology, Jorhat 785006, Assam, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India. Electronic address:
Article Synopsis
- - The study introduces a novel method using Mesua Ferrea Carbon Dots (MFCDs) in biopolymeric films to detect harmful heavy metal ions like Fe, Cd, Hg, Pb, and Cu through optical techniques.
- - A combination of Na-Alginate and Agarose biopolymers was utilized to create an effective matrix, enhancing properties like hydrophilicity and UV absorption, which are crucial for identifying these metals.
- - Detailed optical analyses revealed that the MFCDs-coated films can effectively quench the fluorescence of specific metal ions, showcasing their potential as a sensitive, cost-effective, and environmentally friendly alternative for heavy metal detection.
Enhancing catalytic efficiency of GAO-5F from Fusarium odoratissimum and its application in development of a polyaldehyde crosslinked gelatin-based edible packaging film.
Int J Biol Macromol
December 2024
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China; Laboratory for Marine Science and Technology, Qingdao Marine Science and Technology Center, Qingdao, Shandong 266237, China. Electronic address:
Galactose oxidase has long captured the interest of the biocatalysis and biotechnology communities due to its unique catalytic characteristics and versatility with various substrates. In our previous studies, we demonstrated that galactose oxidase GAO-5F from Fusarium odoratissimum can oxidize agarose to produce a polyaldehyde polymer, which can be further crosslinked with gelatin to produce food packaging films. Despite its commendable catalytic performance, GAO-5F falls short of meeting the requirements for industrial applications.
View Article and Find Full Text PDFPreclinical approaches to evaluate bark extract loaded FDOFs using osteoarthritis models.
Heliyon
October 2024
Pharmacy Department, Osmania University, Hyderabad, Telangana, 500007, India.
Osteoarthritis (OA) is one of the biggest global health issues, affecting two thirds of the population and cannot be fully treated to return normal function or relieve joint discomfort. Oral fast-dissolving films offer a high medication loading capacity, targeted distribution, and increasing bioavailability. The current research explores the in vitro and in vivo efficacy of oral fast-dissolving film formulations containing bark extract.
View Article and Find Full Text PDFFlexible surface acoustic wave technology for enhancing transdermal drug delivery.
Drug Deliv Transl Res
August 2024
Faculty of Engineering and Environment, Northumbria University, Newcastle Upon Tyne, Newcastle, NE1 8ST, UK.
Transdermal drug delivery provides therapeutic benefits over enteric or injection delivery because its transdermal routes provide more consistent concentrations of drug and avoid issues of drugs affecting kidneys and liver functions. Many technologies have been evaluated to enhance drug delivery through the relatively impervious epidermal layer of the skin. However, precise delivery of large hydrophilic molecules is still a great challenge even though microneedles or other energized (such as electrical, thermal, or ultrasonic) patches have been used, which are often difficult to be integrated into small wearable devices.
View Article and Find Full Text PDFConfined Gel-Electromembrane Extraction of Oligonucleotides.
Anal Chem
August 2024
Department of Forensic Medicine, Huazhong University of Science and Technology, Wuhan 430030, China.
Gel-electromembrane extraction (G-EME) is an increasingly popular green variant of electromembrane extraction (EME). However, the electroendosmosis (EEO) flow associated with G-EME greatly limits the development of this technology. To address this challenge, the current study proposed the concept of confined G-EME (CG-EME), and a three-dimensional-printed modular device was elaborately designed to realize this concept.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!