Effect of Absolute From L. Flower on Wound Healing in Keratinocytes.

Background: Proliferation and migration of keratinocytes are essential for the repair of cutaneous wounds. L. has been used in Asian medicine; however, research on keratinocytes is inadequate.

Objective: To establish the dermatological properties of absolute from L. flower (HSF) and to provide fundamental research for alternative medicine.

Materials And Methods: We identified the composition of HSF absolute using gas chromatography-mass spectrometry analysis. We also examined the effect of HSF absolute in HaCaT cells using the XTT assay, Boyden chamber assay, sprout-out growth assay, and western blotting. We conducted an wound healing assay in rat tail-skin.

Results: Ten major active compounds were identified from HSF absolute. As determined by the XTT assay, Boyden chamber assay, and sprout-out growth assay results, HSF absolute exhibited similar effects as that of epidermal growth factor on the proliferation and migration patterns of keratinocytes (HaCaT cells), which were significantly increased after HSF absolute treatment. The expression levels of the phosphorylated signaling proteins relevant to proliferation, including extracellular signal-regulated kinase 1/2 (Erk 1/2) and Akt, were also determined by western blot analysis.

Conclusion: These results of our and ex-vivo studies indicate that HSF absolute induced cell growth and migration of HaCaT cells by phosphorylating both Erk 1/2 and Akt. Moreover, we confirmed the wound-healing effect of HSF on injury of the rat tail-skin. Therefore, our results suggest that HSF absolute is promising for use in cosmetics and alternative medicine.

Summary: L. flower absolute increases HaCaT cell migration and proliferation. L. flower absolute regulates phosphorylation of ERK 1/2 and Akt in HaCaT cell.Treatment with L. flower induced sprout outgrowth.The wound in the tail-skin of rat was reduced by L. flower absolute HSF: L. flower, Erk 1/2: extracellular signal-regulated kinase 1/2, EGF: epidermal growth factor, GC/MS: gas chromatography-mass spectrometry, DMEM: dulbecco's modified eagle medium, FBS: fetal bovine serum, BSA: bovine serum albumin, p-Akt: phosphorylation of Akt, p-Erk 1/2: phosphorylation of Erk 1/2.