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Formalin and 2.5% Glutaraldehyde/2% Paraformaldehyde in 0.1 M Cacodylate Buffer Inactivation Protocols to Ensure the Proper Fixation of Positive Sense RNA Viruses and Genomic Material Prior to Removal from Containment.
Methods Protoc
December 2024
Virology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, MD 21702, USA.
Recommendations released by the CDC in 2023 address the need to demonstrate that the RNA genome of positive-strand RNA viruses is inactivated in addition to viral particles. This recommendation is in response to the similarities between host mRNA and the viral genome that allow the viral RNA to be used as a template by host replication mechanisms to produce infectious viruses; therefore, there is concern that through artificial introduction into host cells, active positive-strand RNA genomes can be utilized to produce infectious viruses out of a containment facility. Utilizing 10% formalin for 7 days or 2.
View Article and Find Full Text PDFDesign of a Freely Accessible Web Application (Instrument for the Measurement of Balance in Primary Education, IMEP) for the Assessment of Static and Dynamic Balance in Children Aged 6-9 Years Based on Force Platforms.
J Funct Morphol Kinesiol
December 2024
Department of Sports Management and Physical Activity, Catholic University of Valencia, 46900 Valencia, Spain.
The proper development of balance is essential in the acquisition of a correct physical condition, as well as in the evolutionary follow-up at early ages, and its periodic evaluation is very relevant in the educational environment. : The objective of this research was to design an accessible web application for static and dynamic balance assessment, based on a force platform and motion analysis software. : The Single leg balance test (SLB), Tandem balance test (TBT), and Y balance test (YBT) were performed on a sample of 75 children aged 6 to 9 years.
View Article and Find Full Text PDFImmunolocalization of Na(+)-Dependent Glucose Co-Transporters in Chicken Kidneys in Norm and During T-2 Mycotoxicosis (Primary Study).
Curr Issues Mol Biol
December 2024
Faculty of Veterinary Medicine, Ss. Cyril & Methodius University in Skopje, 1000 Skopje, North Macedonia.
The kidney plays an essential role in the proper homeostasis of glucose. In the kidney, glucose transport is carried out across cell membranes by two families of glucose transporters-facilitated diffusion glucose transporters (GLUTs) and Na(+)-dependent glucose co-transporters (SGLT family). Among the transporters, sodium-dependent glucose co-transporters play a major role in the kidney's ability to reabsorb glucose.
View Article and Find Full Text PDFReference ranges for complete blood count in children and adolescents with Down syndrome.
Front Pediatr
December 2024
Pediatrics, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy.
Introduction: Down syndrome (DS) is linked to unique hematopoietic characteristics that affect complete blood count (CBC) parameters. Accurate reference ranges are essential for proper CBC interpretation in this population.
Methods: This retrospective study analyzed 2,627 CBCs from 481 DS patients, aged 31 days to 18 years, at a tertiary care center in Italy.
Protein adsorption to poly(ethylenimine)-modified Sepharose FF: IX. Further studies on counterion effects and behavior in therapeutic protein separation.
J Chromatogr A
December 2024
Department of Biochemical Engineering, School of Chemical Engineering and Technology and Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin 300350, PR China. Electronic address:
Our previous studies on protein adsorption onto anion-exchangers of poly(ethylenimine) (PEI)-grafted Sepharose FF (PEI-Sepharose) proved their significantly improved performance over the commercial nongrafting anion-exchangers such as Q Sepharose FF, and it was found the protein adsorption behavior on PEI-Sepharose was more sensitive to counterions (Cl, SCN, HPO and SO). However, the complicated role of counterions has not been well interpreted due to their distinct chemical and physical characteristics. Thus, we have further studied the counterion effects by adding two halide ions (F and Br) to explore the effects of the three halide ions on bovine serum albumin adsorption and the results were compared with previous data.
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