Objectives: To analyse the antineoplastic activity of fractions derived from the hydroalcoholic extract of Euterpe oleracea Mart. seed in the MCF-7 cell line and to identify the compounds responsible for the antineoplastic action.
Methods: Cells were treated with 10, 20, 40 and 60 μg/ml with the hexane, chloroform and ethyl acetate fraction (EAF) of the hydroalcoholic extract of açaí seed, for 24 and 48 h. After treatment, cell viability was measured using MTT assay and cell death was assessed using the Annexin-Pi assay. The most cytotoxic fraction under study was analysed by mass spectrometry using an electrospray ionization source and a cyclotron analyser coupled to a Fourier transform. Data were analysed statistically by analysis of variance (ANOVA) or by Student's t-test, where appropriate.
Key Findings: All fractions caused significant reduction in the cell viability, but the EAF was the most cytotoxic (P < 0.001). It was observed the absence of significant annexin staining but increase Pi staining (P < 0.001). The EAF is composed of epicatechin, proanthocyanidin A and trimeric and tetrameric procyanidins.
Conclusions: In this study, we demonstrated that EAF was the most effective fraction in reducing cell viability and causing necroptosis in the MCF-7 cell.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/jphp.12679 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Wild or Reared? Jellyfish as a Potential Biofactory.
Mar Drugs
January 2025
Consiglio Nazionale delle Ricerche, Istituto di Scienze delle Produzioni Alimentari, (CNR-ISPA)-Lecce, Via Monteroni, 73100 Lecce, Italy.
The zooxanthellate jellyfish (Forsskål, 1775), a Lessepsian species increasingly common in the western and central Mediterranean Sea, was investigated here to assess its potential as a source of bioactive compounds from medusa specimens both collected in the wild (the harbor of Palermo, NW Sicily) and reared under laboratory-controlled conditions. A standardized extraction protocol was used to analyze the biochemical composition of the two sampled populations in terms of protein, lipid, and pigment contents, as well as for their relative concentrations of dinoflagellate symbionts. The total extracts and their fractions were also biochemically characterized and analyzed for their in vitro antioxidant activity to quantify differences in functional compounds between wild and reared jellyfish.
View Article and Find Full Text PDFEffects on the Depressive-like Behavior of MPTP-Exposed Female Rats: Apoptosis and α-Synuclein Modulation.
Brain Sci
January 2025
Research Group on Biochemistry and Toxicology in Eukaryotes, Federal University of Pampa-Campus Uruguaiana, Uruguaiana 97500-970, RS, Brazil.
Parkinson's disease (PD) is a neurodegenerative disorder marked by motor deficits and non-motor symptoms, such as depression, which are associated with dopaminergic loss and α-synuclein aggregation in the brain. This study investigated the neuroprotective effects of a hydroalcoholic extract of the purple fruit of (PFEU) on motor ability and depressive-like behaviors in a PD model induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in female Wistar rats. Rats received intranasal administration of MPTP or vehicle, followed by 14 days of oral administration of PFEU (300 or 2000 mg/kg, administered once daily) or vehicle.
View Article and Find Full Text PDFIntegrated biotechnological process based on submerged fermentation and sonotrode extraction as a valuable strategy to obtain phenolic enriched extracts from moringa leaves.
Food Res Int
February 2025
Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Centre, University of Granada, Avd. del Conocimiento s/n, 18100 Granada, Spain; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avd. Fuentenueva s/n, 18071 Granada, Spain. Electronic address:
The present study explored the influence of submerged fermentation with different lactic acid bacteria (LAB) as a pre-treatment of moringa leaves before the extraction of phenolic compounds via sonotrode. The sonotrode extraction was optimized using a Box-Behnken design and the optimal conditions found were: 25 % ethanol, 5 min and 100 % amplitude. Folin-reacting substances and antioxidant activity were measured on hydroalcoholic extracts obtained from fermented moringa leaves and, individual phenolic compounds were determined by HPLC-ESI-TOF-MS for the first time.
View Article and Find Full Text PDFNutritional value, phytochemical content, and pharmacological screening of L. fruits.
Nat Prod Res
January 2025
Department of Applied Science, Faculty of Engineering & Technology, Gurukula Kangri (Deemed to be University), Haridwar, India.
The present study aimed to evaluate the nutrition value, phytochemical content, and diverse pharmacological activities of different solvent extracts of L. fruit. Among all, the hydro-alcoholic extract showed high DPPH and ABTS radical scavenging activities with IC values of 82.
View Article and Find Full Text PDFInsecticidal effects of Sargassum vulgare and Caulerpa racemosa extracts on Aedes aegypti.
Parasitol Int
January 2025
Department of Fundamental Chemistry, Center for Natural Sciences, Federal University of Pernambuco, Av. Jorn. Aníbal Fernandes, s/n - Cidade Universitária, 50740-560 Recife, Pernambuco, Brazil. Electronic address:
Dengue is a viral disease present in many regions of the world. Aedes aegypti transmits it, and the most effective way to eliminate the mosquito is during the larval stage. Seaweeds possess metabolites with insecticidal properties, making them potential sources of new larvicides and viable alternatives to synthetic products used to control insect vectors of diseases.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!