AI Article Synopsis
- This study developed a reliable method to measure tryptamine even when other similar compounds, like serotonin and l-tryptophan, are present.
- The method combines specially designed microscale sorbents and high-performance liquid chromatography with fluorimetric detection, achieving high effectiveness indicated by an imprinting factor of 15.4 to 18.6.
- Validation tests showed that the method can accurately recover 98.7 to 107.0% of tryptamine from complex samples like bovine serum albumin, with very low detection limits enabling measurement in human cerebrospinal fluid.
Article Abstract
This study presents a validated strategy for the determination of tryptamine in the presence of its competitors, which involves the molecularly imprinted solid-phase extraction combined with high-performance liquid chromatography coupled with fluorimetric detection. Tryptamine-imprinted microscale sorbent was produced from 4-vinylbenzoic acid and ethylene glycol dimethacrylate in methanol by precipitation polymerization, and its imprinting factor was equal to 15.4 in static experiments or 18.6 in dynamic binding experiments. The method for tryptamine determination in the presence of serotonin and l-tryptophan was validated using a complex matrix of bovine serum albumin yielding the recoveries of tryptamine that ranged between 98.7 and 107.0%. Very low limits of detection and limits of quantification for tryptamine (19.9 and 60.3 nmol/L, respectively) allow the quantification of tryptamine in human cerebrospinal fluid in the presence of tryptophan and serotonin.
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| http://dx.doi.org/10.1002/jssc.201601349 | DOI Listing |
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