Hepatocellular carcinoma (HCC) cells often invade the portal venous system and subsequently develop into portal vein tumour thrombosis (PVTT). Long noncoding RNAs (lncRNAs) have been associated with HCC, but a comprehensive analysis of their specific association with HCC metastasis has not been conducted. Here, by analysing 60 clinical samples' RNA-seq data from 20 HCC patients, we have identified and characterized 8,603 candidate lncRNAs. The expression patterns of 917 recurrently deregulated lncRNAs are correlated with clinical data in a TCGA cohort and published liver cancer data. Matched array data from the 60 samples show that copy number variations (CNVs) and alterations in DNA methylation contribute to the observed recurrent deregulation of 235 lncRNAs. Many recurrently deregulated lncRNAs are enriched in co-expressed clusters of genes related to cell adhesion, immune response and metabolic processes. Candidate lncRNAs related to metastasis, such as HAND2-AS1, were further validated using RNAi-based loss-of-function assays. Thus, we provide a valuable resource of functional lncRNAs and biomarkers associated with HCC tumorigenesis and metastasis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316832 | PMC |
| http://dx.doi.org/10.1038/ncomms14421 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
NGF-mediated crosstalk: unraveling the influence of metabolic deregulation on the interplay between neural and pancreatic cancer cells and its impact on patient outcomes.
Front Pharmacol
December 2024
Medical Oncology Unit, University Hospital of Parma, Parma, Italy.
Neural invasion is one of the most common routes of invasion in pancreatic cancer and it is responsible for the high rate of tumor recurrence after surgery and the pain generation associated with pancreatic cancer. Several molecules implicated in neural invasion are also responsible for pain onset including NGF belonging to the family of neutrophins. NGF released by cancer cells can sensitize sensory nerves which in turn results in severe pain.
View Article and Find Full Text PDFDOT1L mediates stem cell maintenance and represents a therapeutic vulnerability in cancer.
Cancer Res
December 2024
Georgetown University, Washington, DC, United States.
Tumor-initiating cancer stem cells (CSC) pose a challenge in human malignancies since they are largely treatment resistant and can seed local recurrence and metastasis. Epigenetic mechanisms governing cell fate decisions in embryonic and adult stem cells are deregulated in CSCs. This review focuses on the methyltransferase DOT1L, which methylates H3K79 and is a key epigenetic regulator governing embryonic organogenesis and adult tissue stem cell maintenance.
View Article and Find Full Text PDFTargeting Epigenetic Dysregulations in Head and Neck Squamous Cell Carcinoma.
J Dent Res
December 2024
Department of Genetics and Development, Columbia University Irving Medical Center, New York, NY, USA.
Head and neck squamous cell carcinoma (HNSCC) is one of the deadliest human cancers, with the overall 5-year survival rate stagnating in recent decades due to the lack of innovative treatment approaches. Apart from the recently Food and Drug Administration-approved epidermal growth factor receptor inhibitor and immune checkpoint inhibitor, alternative therapeutic strategies that target epigenetic abnormalities, an emerging cancer hallmark, remain to be fully explored. A pathological epigenetic landscape, characterized by widespread reprogramming of chromatin modifications such as DNA methylation and histone modifications, which drives transcription deregulation and genome reorganization, has been extensively documented in numerous cancers, including HNSCC.
View Article and Find Full Text PDFDGKH-mediated phosphatidic acid oncometabolism as a driver of self-renewal and therapy resistance in HCC.
Hepatology
November 2024
School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Article Synopsis
- Hepatocellular carcinoma (HCC) has metabolic abnormalities that help cancer cells grow and resist treatment, making it vital to study new metabolic factors linked to these issues for better understanding and potential therapy advancements.
- An analysis of cancer data revealed that a particular gene module correlates with advanced HCC disease and the maintenance of cancer stem cells, leading to the identification of 361 deregulated genes, with diacylglycerol kinase eta (DGKH) being a key player in promoting aggressive cancer traits.
- DGKH's elevated levels in tumors make patients less responsive to treatments, and its action involves enhancing mTOR signaling through phosphatidic acid production; targeting DGKH may improve treatment outcomes
Associations of the Expression Levels and Risk Variants of CDKN2B-AS1 Long Noncoding RNA With the Susceptibility and Progression of Prostate Cancer.
J Cell Mol Med
December 2024
Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Article Synopsis
- Genetic variants of long noncoding RNAs, specifically SNPs in CDKN2B-AS1, are linked to cancer risk and progression, with a focus on prostate cancer (PCa) in this study.
- Five SNPs were analyzed in a case-control study of 695 PCa patients and 695 healthy individuals, revealing that the rs1333048 variant increases the risk of developing aggressive tumors.
- Higher expression levels of CDKN2B-AS1 were found in individuals with the C allele of rs1333048, which correlated with larger tumors and worse outcomes, suggesting that this variant could influence the progression of prostate cancer.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!