AI Article Synopsis
- Serotonin (5-HT) is crucial for regulating milk volume during lactation and may also impact infant growth, but there’s limited data on its levels in human breast milk.
- Researchers developed a high-performance liquid chromatography method to measure 5-HT concentrations in breast milk samples from four healthy Japanese women.
- The method showed high reliability, with 5-HT concentrations in the milk ranging from 11.1 to 51.1 ng/ml and a recovery rate between 96.1% and 101%.
Article Abstract
Serotonin (5-hydroxytryptamine, 5-HT) plays an important role in milk volume homeostasis in the mammary gland during lactation; 5-HT in milk may also affect infant development. However, there are few reports on 5-HT concentrations in human breast milk. To address this issue, we developed a simple method based on high-performance liquid chromatography with fluorescence detection (HPLC-FD) for measuring 5-HT concentrations in human breast milk. Breast milk samples were provided by four healthy Japanese women. Calibration curves for 5-HT in each sample were prepared with the standard addition method between 5 and 1000 ng/ml, and all had correlation coefficients >0.999. The recovery of 5-HT was 96.1%-101.0%, with a coefficient of variation of 3.39%-8.62%. The range of 5-HT concentrations estimated from the calibration curves was 11.1-51.1 ng/ml. Thus, the HPLC-FD method described here can effectively extract 5-HT from human breast milk with high reproducibility.
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| http://dx.doi.org/10.1016/j.bbrc.2017.02.027 | DOI Listing |
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