Production of Potent Antimicrobial Compounds from Associated with Fresh Water Sediment.

The genus under phylum actinobacteria has been recognized as a prolific source for the production of bioactive secondary metabolites. An actinobacterial strain designated as DST103 isolated from a wetland fresh water sediment of Tamdil Lake, Mizoram, Northeast, India was identified as (KY287599) using 16SrRNA gene sequencing which shares 99.87% sequence similarity with NRRL B-2570 . The strain showed broad spectrum antimicrobial activities against Gram negative bacteria ( MTCC 739 and MTCC 2453), Gram positive bacteria ( NCIM 2170 and MTCC 96) and yeast pathogen MTCC 3017). The methanolic extract of the strain DST103 exhibited highest antimicrobial activity against (IC = 2.10 μg/mL) and minimum activity against (IC = 43.63 μg/mL). Five antibiotics [trimethoprim (18 μg/g), fluconazole (6 μg/g), ketoconazole (18 μg/g), nalidixic acid (135 μg/g), and rifampicin (56 μg/g)] were detected and quantified using ultra-performance liquid chromatography (UPLC-ESI-MS/MS). Further, biosynthetic potential genes [polyketide synthases type II, non-ribosomal peptide synthetases, and aminodeoxyisochorismate synthase (E)] were also detected in strain DST103 which may possibly be responsible for the production of antimicrobial compounds. Additionally, gas chromatography-mass spectrometry analysis showed the presence of four volatile compounds which might be responsible for their diverse biological activity. The present study revealed the presence of bioactive compounds in strain DST103, which may be a promising resource for the discovery of novel bioactive metabolites against wide range of pathogens.