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Structure of Methylobacterium extorquens malyl-CoA lyase: CoA-substrate binding correlates with domain shift. | LitMetric

Structure of Methylobacterium extorquens malyl-CoA lyase: CoA-substrate binding correlates with domain shift.

Acta Crystallogr F Struct Biol Commun

Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.

Published: February 2017

AI Article Synopsis

Article Abstract

Malyl-CoA lyase (MCL) is an Mg-dependent enzyme that catalyzes the reversible cleavage of (2S)-4-malyl-CoA to yield acetyl-CoA and glyoxylate. MCL enzymes, which are found in a variety of bacteria, are members of the citrate lyase-like family and are involved in the assimilation of one- and two-carbon compounds. Here, the 1.56 Å resolution X-ray crystal structure of MCL from Methylobacterium extorquens AM1 with bound Mg is presented. Structural alignment with the closely related Rhodobacter sphaeroides malyl-CoA lyase complexed with Mg, oxalate and CoA allows a detailed analysis of the domain motion of the enzyme caused by substrate binding. Alignment of the structures shows that a simple hinge motion centered on the conserved residues Phe268 and Thr269 moves the C-terminal domain by about 30° relative to the rest of the molecule. This domain motion positions a conserved aspartate residue located in the C-terminal domain in the active site of the adjacent monomer, which may serve as a general acid/base in the catalytic mechanism.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297927PMC
http://dx.doi.org/10.1107/S2053230X17001029DOI Listing

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