AI Article Synopsis
- - This study employs TALE nucleases (TALENs) to successfully target and edit the Vasa gene in chicken primordial germ cells (PGCs), which play a crucial role in germ cell development in birds.
- - The researchers achieved high efficiency (8.1%) in using TALENs for homology-directed repair of the Vasa locus on the Z sex chromosome, and also created significant genetic deletions of 30 kb.
- - The modified PGCs were capable of producing offspring, but knockout chickens experienced the loss of PGCs during meiosis, resulting in sterility for adult females, demonstrating the effective targeting potential of TALENs in avian genetics.
Article Abstract
In this work we use TALE nucleases (TALENs) to target a reporter construct to the () locus in chicken primordial germ cells (PGCs). Vasa is a key germ cell determinant in many animal species and is posited to control avian germ cell formation. We show that TALENs mediate homology-directed repair of the locus on the Z sex chromosome at high (8.1%) efficiencies. Large genetic deletions of 30 kb encompassing the entire locus were also created using a single TALEN pair. The targeted PGCs were germline competent and were used to produce null offspring. In knockout chickens, PGCs are initially formed but are lost during meiosis in the developing ovary, leading to adult female sterility. TALEN-mediated gene targeting in avian PGCs is therefore an efficient process.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374353 | PMC |
| http://dx.doi.org/10.1242/dev.145367 | DOI Listing |
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