; a Recently Detected Virus Infecting Alfafa () in Central Saudi Arabia.

Plant Pathol J

Department of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia.

Published: February 2017

A survey was conducted to determine the status of (LTSV) in three high-yielding alfalfa regions in central Saudi Arabia (Riyadh, Qassim, and Hail) during 2014. Three hundred and eight symptomatic alfalfa, and seven samples were collected. DAS-ELISA indicated that 59 of these samples were positive to LTSV. Two isolates of LTSV from each region were selected for molecular studies. RT-PCR confirmed the presence of LTSV in the selected samples using a specific primer pair. Percentage identity and homology tree comparisons revealed that all Saudi isolates were more closely related to each other but also closely related to the Canadian isolate-JQ782213 (97.1-97.6%) and the New Zealand isolate-U31286 (95.8-97.1%). Comparing Saudi isolates of LTSV with ten other sobemoviruses based on the coat protein gene sequences confirmed the distant relationship between them. Eleven out of fourteen plant species used in host range study were positive to LTSV. This is the first time to document that , , , and are new host plant species for LTSV and that being a good propagative host for it.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291397PMC
http://dx.doi.org/10.5423/PPJ.OA.06.2016.0143DOI Listing

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