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Filename: drivers/Session_files_driver.php
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Filename: controllers/Detail.php
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Function: _error_handler
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Filename: controllers/Detail.php
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Function: _error_handler
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Filename: controllers/Detail.php
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Function: _error_handler
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Filename: controllers/Detail.php
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Filename: models/Detail_model.php
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Function: strpos
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Function: insertAPISummary
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Filename: controllers/Detail.php
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Filename: controllers/Detail.php
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Function: _error_handler
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Filename: controllers/Detail.php
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Filename: controllers/Detail.php
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File: /var/www/html/application/controllers/Detail.php
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Filename: controllers/Detail.php
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Filename: controllers/Detail.php
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Function: _error_handler
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Filename: controllers/Detail.php
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Filename: controllers/Detail.php
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Filename: controllers/Detail.php
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Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
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Background: Testing for hemoglobin A1C (HbA1C) is useful for following up on glycemic control in diabetic patients as well as in pregnant women.
Methods: A pregnant Chinese woman appeared for regular pregnancy check-ups. While monitoring blood glucose, an HbA1c test was initially performed in the pregnant Chinese woman using a CE-HPLC assay and capillary electrophoresis. Then acid gel Hb electrophoresis, a reverse dot-blot (RDB) assay, gap-PCR and DNA sequencing were applied to further evaluate the Hb variants.
Results: The CE-HPLC system has shown that the percentage of blood HbA1c was 5.4%, while hemoglobin electrophoresis of HbA2 and HbA0 were 1.6% and 88.7%, respectively. The capillary electrophoresis measurement results were 6.4% HbA1c, 0.4% HbA2, and 84.1% HbA0. HbA2 and HbA were 0.8% and 89.3%, respectively. Further gap-PCR, the reverse dot-blot (RDB) assay, and DNA sequencing showed that the pregnant woman had HbH-CS (αα CS/--) (1800 bp and 1300 bp) and β/β.
Conclusions: Our study demonstrates that hemoglobin variants, such as HbH-CS can affect capillary electrophoresis results. An HbH-CS diagnosis in a pregnant woman is clinically significant. Laboratories should be cautious in using the CE-HPLC assay to evaluate HbA1c results in the presence of hemoglobin variants. Our findings highlight the strong discriminatory power of capillary electrophoresis for various Hb variants.
Download full-text PDF |
Source |
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http://dx.doi.org/10.7754/Clin.Lab.2015.151014 | DOI Listing |
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