Background: The performance of a new intermediate-resolution method using a PCR-Luminex platform and LABType® SSO A, B DRB1 kits as an HLA typing method for the cord blood (CB) registry of the Korean population was investigated.
Methods: A total of 1,413 cord blood units (CBUs) were enrolled - 1,382 from Koreans and 31 from non-Koreans or mixed-ancestry individuals. HLA-A, -B, and -DRB1 typing was performed using the LABType® SSO typing kits. HLA typing with the DNA method and 2-digit results are mandatory for the public CB bank in Korea according to the "CB Act."
Results: The proportions of ambiguous results in the 2-digit assignment were 14.6% (206/1,413) and 14.8% (205/ 1,382) among the total subjects and the Korean donors, respectively. In the 2-digit resolution, 3 different HLA-A types (69 CBUs), 31 HLA-B types (124 CBUs), and 3 HLA-DRB1 types (13 CBUs) showed ambiguous results. The 'most probable type' to the ambiguous results based on the reported Korean HLA allele frequencies were able to be assigned. The most probable results were 100% consistent with the confirmed types as determined by the HD kits (DRB1) and additional PCR-SBT or PCR-SSP tests (A and B). Luminex technology is more automated and less labor intensive than the conventional SSO typing method, and the results are less affected by differences between inspectors.
Conclusions: Although it is not satisfactory as a sole confirmation test and cannot be used as a replacement for the PCR-SBT test, the combination of Luminex technology with LABType® SSO kits and population frequency data provides a proper typing platform that can be used as a qualifying test for CB registries.
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http://dx.doi.org/10.7754/Clin.Lab.2016.160101 | DOI Listing |
Clin Pract
January 2025
Laboratory of immunology and HLA, Center of Clinical Research, Mohammed VI University Hospital, Marrakech 43150, Morocco.
Many factors contribute to the development and the progression of Multiple Sclerosis (MS), including Human Leukocyte Antigen (HLA) molecules. Some of them are considered as predisposing, like DRB1*15, DRB1*13, DRB1*03, DRB1*04, DQB1*06, DQB1*02, while HLA A2, HLA B44, DRB1*11, and DRB1*12 are rather considered as protective. Data about such associations in the Moroccan population remain unknown.
View Article and Find Full Text PDFBiomedica
December 2024
Servicio de Reumatología e Inmunología, Hospital Militar Central, Bogotá, D. C., Colombia; Grupo de Inmunología Clínica Aplicada, Hospital Militar Central, Universidad Militar Nueva Granada, Bogotá, D. C., Colombia; Grupo de Inmunología Celular y Molecular - InmuBo, Universidad El Bosque, Bogotá, D. C., Colombia.
Introduction: Psoriatic arthritis is a complex disease, and human leukocyte antigens (HLA) are key to its development. Latin America and, specifically, Colombia, has scarce data about patients with psoriatic arthritis.
Objective: To describe the genotypic, allelic and haplotypic frequency of HLA alleles in psoriatic arthritis and associate them with clinical variables.
Enhancement of security, personalization, and safety in advanced transportation systems depends on driver identification. In this context, this work suggests a new method to find drivers by means of a Random Forest model optimized using the osprey optimization algorithm (OOA) for feature selection and the salp swarm optimization (SSO) for hyperparameter tuning based on driving behavior. The proposed model achieves an accuracy of 92%, a precision of 91%, a recall of 93%, and an F1-score of 92%, significantly outperforming traditional machine learning models such as XGBoost, CatBoost, and Support Vector Machines.
View Article and Find Full Text PDFJoint Bone Spine
January 2025
Marmara University, Institute of Health Sciences, Department of Medical Biology and Genetics, Istanbul, Turkey; Department of Medical Biology, School of Medicine, Marmara University, Istanbul, Turkey. Electronic address:
Objective: Bamboo spine is the most severe complication of Axial spondyloarthritis (AxSpA). This study aims to address whether haplotypes of endoplasmic reticulum aminopeptidase (ERAP)1 and ERAP2 single nucleotide polymorphisms (SNPs), previously associated with AXSPA, are associated with the development of bamboo spine in an AxSpA cohort.
Methods: The study included 192 patients with AxSpA followed in MARS (MARmara Spondyloarthritis) clinic and fulfilling the modified New York criteria.
J Am Coll Surg
January 2025
Department of Surgery, New Hanover Regional Medical Center, Novant Health, Wilmington, NC.
Background: Patients with large pannus and ventral hernias may benefit from undergoing panniculectomy performed concurrently with open ventral hernia repair (VHR-PAN). However, there has been concerns related to increase surgical site occurrences when adding a panniculectomy. This study aims to evaluate outcomes of open VHR with and without panniculectomy using a large hernia specific database.
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