Coconut oil sludge and oil cake was utilized as carbon source for biosurfactant production by Pseudomonas aeruginosa AMB AS7. The results of optimization study revealed that 1.5% (w/v) of coconut oil cake, 2% (w/v) of coconut oil sludge, pH 7.2, 37 °C, and 120 rpm were the optimum conditions for biosurfactant production. The yield coefficient of biosurfactant on biomass (Y ) was 1.29 g/g. Besides, the results indicated that aeration of 0.5 vvm and agitation of 450 rpm in bioreactor resulted in high volumetric productivity of biosurfactant (r ) and specific product formation rate (q ) of 0.115 g/(L h) and 0.0131 g/(g h), respectively in medium containing 2% (w/v) coconut oil sludge. The maximum biosurfactant concentration of 5.53 g/L was obtained during 60 h of cultivation. The emulsification index (EI) against coconut oil was found to be 88.42 ± 0.5%, and cell surface hydrophobicity of P. aeruginosa AMB AS7 was obtained 32.4 ± 0.9%. FTIR and GC-MS analysis revealed that the biosurfactant is rhamnolipid with anionic charge. The critical micelle concentration (CMC) of rhamnolipid was found to be 50 mg/L. It was found that 66.95% of chromium from aqueous solution can be removed using rhamnolipid at its CMC.
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http://dx.doi.org/10.1007/s12010-017-2431-6 | DOI Listing |
J Chromatogr A
January 2025
Analytical Chemistry Laboratory, Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés 2, Gembloux 5030, Belgium. Electronic address:
The analysis of mineral oil aromatic hydrocarbons (MOAH) in vegetable oils is currently associated with high uncertainty due to various factors ranging from sample preparation to data interpretation. One significant factor is the coelution of biogenic compounds of terpenic origin with the MOAH fraction during chromatographic analysis. The common purification method is epoxidation, a chemical reaction that changes the polarity of the interferences, allowing their separation from MOAH.
View Article and Find Full Text PDFHeliyon
July 2024
Department of Physics, University of Lucknow, Lucknow 226007, India.
In this paper, the dielectric behaviour of coconut oil within the frequency range 100 kHz to 30 MHz between temperature 30 °C-50 °C has been observed. The measured values of the dielectric constant and dielectric loss show notable variation with frequency and temperature for pure coconut oil. It is noticed that the dielectric constant (՛) and dielectric loss (՛՛) of coconut oil decreases with increasing temperature.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
School of Food Science and Engineering, Wuhan Polytechnic University,Wuhan 430023, China.
Glycosylation can be used to improve the emulsifying properties of protein by covalently binding with sugar. In this study, we prepared coconut protein (CP) -polygalacturonic acid (PA) conjugates by dry-heat method, studied the effect of PA with different molecular weight on the structure and functionality of CP, and characterized the interfacical behavior of CP at the oil-water interface to establish the relationship between interfacial behavior and emulsion stability. The results showed that different molecular weights of PA (28.
View Article and Find Full Text PDFJ Agric Food Chem
January 2025
Department of Agro-Industry, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, 99 Moo 9, Tha Pho, Phitsanulok 65000, Thailand.
This study aimed to evaluate the cholesterol-regulatory effects of lauric-acid-esterified octacosanol (LEO) and oleic-acid-esterified octacosanol (OEO) compared to their unmodified counterparts and to investigate the underlying mechanisms by partially substituting the fat content in obese C57BL/6J mice induced with a high-fat diet (HFD). Rice bran oil and coconut oil were also investigated as they are rich in oleic acid and lauric acid, respectively. The results showed that all supplemented groups significantly inhibited weight gain induced by the HFD, but the groups treated with esterified octacosanol exhibited a more pronounced effect.
View Article and Find Full Text PDFReprod Toxicol
January 2025
Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address:
Saturated fat has been linked to cardiovascular diseases, leading to an increase in polyunsaturated fat consumption. The aim of the present study was to investigate the effects of three fat sources (coconut oil, lard and soybean oil) on metabolic and reproductive parameters in heterogenic mice. Female Swiss mice (5-6 weeks old; n=9/group) were divided into four experimental groups: control (CC), coconut oil (CO), lard (LA), and soybean oil (SO), and were orally given 0.
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