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Effects of endophytes on early growth and ascorbate metabolism in .
Front Plant Sci
December 2024
Department of Plant Biology, Rutgers University, New Brunswick, NJ, United States.
Understanding the early interactions between plants and endophytes will contribute to a more systematic approach to enhancing endophyte-mediated effects on plant growth and environmental stress resistance. This study examined very early growth and ascorbate metabolism after seed treatment of with three different endophytes. The three endophytes used were pb1(Bapb1), (Ml) and SLB4 (SLB4).
View Article and Find Full Text PDFTherapeutic deep eutectic solvent with saponin, optimized through response surface methodology, exert potent in vivo antimicrobial effects against Pseudomonas aeruginosa.
Sci Rep
November 2024
Department of Chemistry, School of Chemical & Biotechnology, Shanmugha Arts, Science, Technology & Research Academy (SASTRA) Deemed University, Thanjavur, 613 401, Tamil Nadu, India.
Therapeutic deep eutectic solvents (THEDES) are a type of deep eutectics that can be used as active pharmaceutical ingredients (APIs) by enhancing the drug bioavailability, by increasing the solubility of APIs in aqueous solutions or by increasing their permeability through biological barriers. In this study, we demonstrate the utility of designer THEDES, based on Quillaja Saponin (SAP), a triterpene natural product to unravel the antimicrobial potential of such THEDES. THEDES were prepared by gently mixing and heating five different α- hydroxy acids- malic, citric, tartaric, glycolic, lactic acids and SAP with water as a third component in the THEDES.
View Article and Find Full Text PDFOn the Billefjorden fault zone in Garmdalen, central Spitsbergen: implications for the mapping of major fault zones during geological fieldwork and for the tectonic history of Svalbard.
Open Res Eur
October 2024
National Agency of Hydrocarbon, Bogota, Cundinamarca, 111321, Colombia.
Background: The present contribution reexamines the geometry of a segment of a presumably long-lived fault in Svalbard, the Balliolbreen Fault segment of the Billefjorden Fault Zone, along which presumably two basement terranes of Svalbard accreted in the early-mid Paleozoic after thousands of kilometers strike-slip displacement.
Methods: We performed structural fieldwork to Billefjorden in central Spitsbergen and interpreted satellite images.
Results: Field observations demonstrate that the Balliolbreen Fault formed as a top-west thrust fault in the early Cenozoic and that weak sedimentary units such as shales of the Lower Devonian Wood Bay Formation and coals of the uppermost Devonian-Mississippian Billefjorden Group partitioned deformation, resulting in significant contrast in deformation intensity between stratigraphic units.
Live cell fluorescence microscopy-an end-to-end workflow for high-throughput image and data analysis.
Biol Methods Protoc
October 2024
Department of Functional Organization of Biomembranes, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic.
Fluorescence microscopy images of biological samples contain valuable information but require rigorous analysis for accurate and reliable determination of changes in protein localization, fluorescence intensity, and morphology of the studied objects. Traditionally, cells for microscopy are immobilized using chemicals, which can introduce stress. Analysis often focuses only on colocalization and involves manual segmentation and measurement, which are time-consuming and can introduce bias.
View Article and Find Full Text PDFFormation and Physicochemical Properties of Freeze-Dried Amyloid-Like Fibrils From Pinto Bean Protein: Amyloid-Like Fibrils From Pinto Bean Protein.
Int J Anal Chem
October 2024
Department of Food Science and Technology, Laser and Biophotonics in Biotechnologies Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
Amyloid nanofibrils are long and thin strands with cross structures associated by hydrogen bonds. These structures can be formed under suitable conditions commonly at low pH and high temperatures. Fibrillated pinto bean protein isolate (FPBPI) was made by heating pinto bean protein at 85°C in an acidic condition while gently stirring at initial protein solution concentrations of 4 mg/mL, 13 mg/mL, and 21 mg/mL.
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