Background: Autologous Conditioned Plasma (ACP) has a wide range of potential uses in modern orthopaedics. The aim of this study was to examine the characteristics of proprietary ACP and compare them with those of ACP produced using a commercially available kit.
Material And Methods: In the hospital laboratory, 20 samples of ACP taken from patients and prepared according to the commercially available kit protocol with a double syringe system were compared with 40 ACP preparations made using disposable sterile equipment available in the hospital.
Results: The mean platelet concentration in the ACP samples prepared according to the Arthrex protocol was 2.02 (range 1.16 to 2.64) times greater than in peripheral blood, while the concentration in the proprietary preparation was 1.61 (range 0.82 to 2.52) times higher. However, the mean platelet density in the proprietary preparation (n = 24) was 1.98 (range 1.48 to 2.52) times that of peripheral blood within 20 minutes of collection, and 1.41 (range 0.84 - 1.87) times after 20 minutes (n = 16). Therefore, the proprietary method of producing ACP is comparable to that of the commercial kit with regard to platelet density (p>0.05).
Conclusion: Using disposable hospital equipment and with a relatively short time between ACP preparation and testing, it is possible to obtain a suitable proprietary platelet-rich preparation comparable to one produced using a commercial system.
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http://dx.doi.org/10.5604/15093492.1230542 | DOI Listing |
Iran J Kidney Dis
December 2024
Pediatric Nephrology Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Introduction: Acute kidney injury (AKI) is a frequent complication after hematopoietic stem cell transplantation (HSCT), with reported incidences ranging from 20-70% within the first 100 days post-transplant. AKI can adversely impact outcomes and survival in this patient population.
Methods: This retrospective study evaluated 110 pediatric patients who underwent HSCT at Mofid Children's Hospital, affiliated with Shahid Beheshti University of Medical Sciences, Tehran, Iran, between 2016-2021.
Front Oncol
December 2024
National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.
Chimeric antigen receptor T-cell (CAR-T) therapy has demonstrated both efficacy and safety in relapsed/refractory diffuse large B-cell lymphoma (DLBCL) patients infected with hepatitis B virus (HBV). However, its applicability in individuals with liver cirrhosis remains largely unexplored due to the potential for unpredictable complications. Here, we report three cases (P1, P2, and P3) of relapsed/refractory DLBCL with HBV-related cirrhosis treated with CAR-T cell infusion.
View Article and Find Full Text PDFStem Cell Res Ther
December 2024
Department of Orthopedic Surgery, Kanazawa Medical University, Kahoku, Ishikawa, 920-0293, Japan.
Background: Adipose-derived stem cells (ADSCs) are widely used in the field of regenerative medicine because of their various functions, including anti-inflammatory effects. ADSCs are considered to exert their anti-inflammatory effects by secreting anti-inflammatory cytokines and extracellular vesicles. Although recent studies have reported that metabolites have a variety of physiological activities, whether those secreted by ADSCs have anti-inflammatory properties remains unclear.
View Article and Find Full Text PDFTumori
December 2024
Department of Pediatrics, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy.
Introduction: The association of acute lymphoblastic leukaemia (ALL) and cystic fibrosis (CF) is rare. We present the case of a paediatric patient affected by CF and refractory B-cell precursor (BCP) ALL, who was treated with combined chimeric antigen receptor T-cells (CAR-T) and allogeneic haematopoietic stem cell transplantation (HSCT).
Case Description: Autologous-CD19 targeting CAR-T allowed to achieve molecular remission and spare chemo-related toxicity.
Cureus
November 2024
Pathology and Laboratory Medicine, University of California Davis Health System, Sacramento, USA.
We present a case of a 36-year-old male found to have a nonseminomatous germ cell tumor (NSGCT) with alpha-fetoprotein levels (AFP) of 737.9 ng/mL and beta-human chorionic gonadotropin (β-HCG) of 692 IU/mL. Pathology analysis after left orchiectomy showed a mixed germ cell tumor with 20% embryonal carcinoma, 20% yolk sac tumor, and 60% teratoma.
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