Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree.

Methods Mol Biol

Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, via A. Ferrata, 9, 27100, Pavia, Italy.

Published: February 2018

Dipeptidyl-peptidase IV is an enzyme involved in a lot of biochemical processes, where it modifies a number of regulatory proteins by removing the terminal peptides by hydrolysis. Here we describe a histochemical method to demonstrate with accuracy and precision its in situ activity on cryostatic section of Wistar rat liver by means of a simultaneous azo-coupling method.

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-4939-6788-9_3DOI Listing

Publication Analysis

Top Keywords

enzyme-histochemistry technique
4
technique visualizing
4
visualizing dipeptidyl-peptidase
4
dipeptidyl-peptidase dpp-iv
4
dpp-iv activity
4
activity liver
4
liver biliary
4
biliary tree
4
tree dipeptidyl-peptidase
4
dipeptidyl-peptidase enzyme
4

Similar Publications

George Gomori's Contributions to Diabetes Research and the Origins of the .

J Histochem Cytochem

December 2024

Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, University of Washington, Seattle, Washington (DGB).

Article Synopsis
  • Gomori was a key figure in enzyme histochemistry for nearly 30 years, pioneering methods to demonstrate enzyme activity, especially phosphatases, esterases, and lipases.
  • He made significant strides in diabetes research by creating histological techniques that effectively stained insulin-secreting B cells in the pancreas, which were crucial for understanding the disease.
  • As a founding member and President of The Histochemical Society, Gomori played a pivotal role in advancing the field, including serving as one of the first Associate Editors for the society's journal.
View Article and Find Full Text PDF

Currently, there are plenty of histochemical methods to classify pig muscle fibers, which confused the naming and classification of muscle fibers. This study aims to analyze the difference and correlation of 6 different histochemical methods and select the most suitable method for muscle fiber classification at the molecular and histomological levels by in-situ RT-PCR and enzyme histochemical methods. Muscle fiber samples, including psoas (PM), semitendinosus (SM) and trapezius muscle (TM), were collected from Large Spotted (LS), Lantang (LT) and Landrace (LR) pigs at their market-ages (LS at 150 d, LT at 210 d, and LR at 150 d).

View Article and Find Full Text PDF

Background: Muscle diseases are of various types, viz., muscular dystrophies, inflammatory myopathies, myotonic disorders, congenital myopathies, and metabolic myopathies. They all present with muscle weakness, be it proximal or distal.

View Article and Find Full Text PDF

Congenital myopathies are defined by early clinical onset, slow progression, hereditary nature and disease-specific myopathological lesions - however, with exceptions - demanding special techniques in regard to morphological diagnostic and research work-up. To identify an index disease in a family requires a muscle biopsy - and no congenital myopathy has ever been first described at autopsy. The nosographic history commenced when - in addition to special histopathological techniques in the earliest classical triad of central core disease, 1956, nemaline myopathy, 1963, and centronuclear myopathy, 1966/67, within a decade - electron microscopy and enzyme histochemistry were applied to unfixed frozen muscle tissue and, thus, revolutionized diagnostic and research myopathology.

View Article and Find Full Text PDF

Background: Idiopathic inflammatory myopathies (IIM), also called autoimmune myositis, are heterogeneous. These include dermatomyositis (DM), inclusion body myositis, immune mediated necrotizing myopathy (IMNM), anti-synthetase syndrome (ASS), and overlap polymyositis. Classification of IIM has evolved from clinical to clinico-pathologic to the recent clinico-sero-pathologic with the discovery of myositis-specific antibodies (MSA) and myositis-associated antibodies.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!