Antifungal Activity of Lactic Acid Bacteria Strains Isolated from Natural Honey against Pathogenic Species.

The role of lactic acid bacteria (LAB) in honey as antifungal activity has received little attention and their mechanism of inhibitory of fungi is not fully understood. In this study, LAB were isolated from honey samples from Malaysia, Libya, Saudi Arabia, and Yemen. Twenty-five isolates were confirmed LAB by catalase test and Gram staining, and were screened for antifungal activity. Four LAB showed inhibitory activity against spp. using the dual agar overlay method. And they were identified as HS isolated from Al-Seder honey, HH isolated from Al-Hanon honey, HC isolated from Tualang honey and HM isolated from Al-Maray honey by the 16S rDNA sequence. The growth of ATCC 2001 was strongly inhibited (>15.0 mm) and (10~15 mm) by the isolates of HH and HM, respectively. The antifungal activity of the crude supernatant (cell free supernatant, CFS) was evaluated using well diffusion method. The CFS showed high antifungal activity against spp. especially The CFS of HH was significantly ( < 0.05) inhibited growth of ATCC 2001, ATCC 2201, and ATCC 750 with inhibitory zone 22.0, 15.6, and 14.7 mm, respectively. While CFS of HM was significantly ( < 0.05) effective against , , and with inhibition zone 17.2, 16.0, and 13.3 mm, respectively. The results indicated that LAB isolated from honey produced compounds which can be used to inhibit the growth of the pathogenic species.