Dengue virus is an arthropod-borne virus transmitted primarily by Aedes mosquitos and is major cause of disease in tropical and subtropical regions. Colloquially known as Dengue Fever, infection can cause hemorrhagic disorders and death in humans and non-human primates. We report a novel electronic biosensor based on a single-walled carbon nanotube network chemiresistive transducer that is functionalized with heparin for low-cost, label-free, ultra-sensitive, and rapid detection of whole dengue virus (DENV). Heparin, an analog of the heparan sulfate proteoglycans that are receptors for dengue virus during infection of Vero cells and hepatocytes, was used for the first time in a biosensor as a biorecognition element instead of traditional antibody. Detection of DENV in viral culture supernatant has similar sensitivity as the corresponding viral titer in phosphate buffer despite the presence of growth media and Vero cell lysate. The biosensor demonstrated sensitivity within the clinically relevant range for humans and infected Aedes aegypti. It has potential application in clinical diagnosis and can improve point-of-care diagnostics of dengue infection.
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http://dx.doi.org/10.1016/j.bios.2017.01.017 | DOI Listing |
Trop Med Infect Dis
January 2025
Department of Research, PMO, Ministry Branch in Makkah Region, Ministry of Health (MOH), Makkah 21955, Saudi Arabia.
Dengue fever is caused by four common serotypes of the dengue virus (DENV-1, DENV-2, DENV-3, and DENV-4). Patients infected with one serotype may develop lifelong serotype-specific protective immunity. However, they remain susceptible to reinfection with the other serotypes, often increasing the risk of severe forms of dengue.
View Article and Find Full Text PDFIJID Reg
March 2025
Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.
Objectives: Arboviruses pose significant public health threats worldwide, with Southeast Asia being a hotspot for these infections. This study aimed to reassess the incidence of dengue, Zika, and chikungunya viruses in patients clinically diagnosed with dengue in East Java, Indonesia in 2023.
Methods: The study included 108 patients admitted to hospitals in Jember, with blood samples collected on admission.
J Arthropod Borne Dis
June 2024
Division of Immunology, Mochtar Riady Institute for Nanotechnology and Medical Science Group, Pelita Harapan University, Tangerang, Indonesia.
Background: The spectrum of dengue infection ranges from asymptomatic or mild to severe disease. The pathogenic mechanisms are not fully understood. A viral infection can induce the neutrophil extracellular traps (NETs), and the excessive NETs lead to increased vascular permeability, coagulopathy, and platelet dysfunction, a hallmark of severe dengue.
View Article and Find Full Text PDFJ Neurol
January 2025
Research, Diagnosis and Reference Center, PAHO/WHO Collaborating Center for the Study of Dengue and Its Control, Institute "Pedro Kourí", Havana, Cuba.
Nanomedicine (Lond)
January 2025
Clinical Laboratory Science Section, Institute of Medical Science Technology, Universiti Kuala Lumpur, Kajang, Selangor, Malaysia.
Phytochemicals are typically natural bioactive compounds or metabolites produced by plants. Phytochemical-loaded nanocarrier systems, designed to overcome bioavailability limitations and enhance therapeutic effects, have garnered significant attention in recent years. The coronavirus disease 2019 (COVID-19) pandemic has intensified interest in the therapeutic application of phytochemicals to combat viral infections.
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