AI Article Synopsis
- The O157:H7 serotype is a significant cause of gastrointestinal infections in developed countries, primarily linked to ruminant animals as natural reservoirs.
- A total of 150 milk samples were collected from dairy farms in Khuzestan over six months and analyzed using selective media and multiplex PCR for the presence of toxigenic O157:H7 strains.
- The study found that while some genes related to O157:H7 were detected in 45 samples, only 2 milk samples tested positive for the toxigenic strain, highlighting the need for strict health standards in dairy production.
Article Abstract
Background And Objectives: The most common serotype of enterohaemorrhagic group or Shiga-toxin-producing is O157:H7. Domestic and wild ruminants are regarded as the main natural reservoirs. O157:H7 serotype is the major cause of gastrointestinal infections in developed countries. In this study was conducted to survey on the toxigenic O157: H7 strains in milk of industrial dairy farms.
Materials And Methods: A total number of 150 milk samples were collected from dairy industry in Khuzestan, over a period of 6 months and were evaluated by cultivation in selective media (CT-SMAC) and multiplex PCR.
Results: Two isolates were identified as using biochemical tests, none of them were toxigenic O157:H7 as determined by multiplex PCR. Using direct PCR on milk samples, 45 samples contained at least one gene of the studied genes in this investigation (, ). With direct PCR, 2 milk samples were positive for toxigenic O157:H7.
Conclusion: O157:H7 is present in this region and so the necessity for strict compliance of health standards is recommended. This is the first study on O157: H7 milk contamination in Khuzestan province. Based on these results, direct PCR is more accurate than indirect PCR.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5277595 | PMC |
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