Transgenes can be introduced into the cells of organotypic slice cultures using different delivery methods, such as biolistic transfection, electroporation, and viral vector-based transduction. These methods produce different patterns of transgene expression. Local injection of recombinant adeno-associated virus (rAAV) produces a small cluster of transgene-expressing neurons around the injection site. Expression in individual cells varies with the distance from the injection site, indicating that many neurons take up several rAAV particles. The serotype and promoter also play a role in transgene expression. Here, we present a protocol for the transduction of previously prepared hippocampal slice cultures with rAVV.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1101/pdb.prot094896 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Basic Science and Pathogenesis.
Alzheimers Dement
December 2024
University of Florida College of Medicine, Gainesville, FL, USA.
Background: The presence of Tau pathology is strongly associated with the clinical symptoms and cognitive decline found in Alzheimer's disease (AD), suggesting that targeting pathological tau may be a more effective therapeutic approach. Microglia have been implicated in tauopathies as their activation is strongly related to the progression of tau phosphorylation and aggregation potentially due to dysfunctional lysosomal activity. Cannabinoid type 2 receptors (CB2) are highly expressed in immune cells and upregulated in activated microglia under conditions of neurologic disease, such as AD.
View Article and Find Full Text PDFBasic Science and Pathogenesis.
Alzheimers Dement
December 2024
Janssen Research & Development, A Division of Janssen Pharmaceutica, Beerse, Belgium, Beerse, Belgium.
Background: Microglial cells have emerged as key players in the pathogenesis of Alzheimer's disease (AD). They act as a first line defense and fulfil a crucial role during brain development and circuit homeostasis. Microglia are involved in the removal of debris, control neural activity, regulate synaptic plasticity, and synapse pruning.
View Article and Find Full Text PDFBasic Science and Pathogenesis.
Alzheimers Dement
December 2024
Centre for Discovery Brain Sciences at the University of Edinburgh, Edinburgh, Scotland, United Kingdom.
Background: Maintaining synaptic health is essential for normal neurological function, yet neurodegenerative diseases like Alzheimer's disease and Progressive Supranuclear Palsy (PSP) exhibit synaptic loss. In these conditions, synaptic loss precedes neuronal degeneration, and the degree of synaptic loss correlates closely with the severity of clinical symptoms. Both Aβ, which accumulates in amyloid plaques in AD, and tau protein which accumulates intracellularly in tauopathies, including AD and PSP, accumulate within synaptic terminals.
View Article and Find Full Text PDFBasic Science and Pathogenesis.
Alzheimers Dement
December 2024
Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Background: Calcineurin, a protein involved in functions such as synaptic plasticity and neuronal survival, plays an important role in the pathophysiology of Alzheimer's disease. This study, randomized, investigated the effects of FK506 (FK), a calcineurin inhibitor, on the behavioral, histological, and biochemical alterations observed in models of neurotoxicity induced by NMDA or Aβ and in a transgenic model for AD, in addition to the organotypic culture model stimulated with NMDA.
Methods: This study involved models for AD to experiment injecting NMDA or Aβ1-42 into the hippocampus of male C57Bl/6 mice aged 8-12 weeks to induce a neurotoxicity model, treating double-transgenic APP/PS1 mice, expressing both mouse/human APP and mutant human PS1, with chronic FK506 for AD, in which, to enable NMDA or Aβ1-42 microinjections, another experiment including a stereotaxic surgery was performed on the C57Bl/6 mice.
Use of dielectric-barrier discharge (DBD) cold plasma for control of bread spoilage fungi.
Int J Food Microbiol
December 2024
Department of Food Engineering and Technology, School of Food Engineering, Universidade Estadual de Campinas, São Paulo, Brazil. Electronic address:
Bread is a greatly consumed bakery product worldwide. Unfortunately, it is an optimal substrate for fungal contamination and deterioration (aw > 0.95), commonly caused by the genera Penicillium, Paecilomyces, and Aspergillus, resulting in significant economic losses.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!