In this study, extracellular metabolites of symbiotic bacteria Xenorhabdus stockiae (KT835471) was employed for the synthesis of silver (XsAgNPs) and gold nanoparticles (XsAuNPs). Synthesized NPs were characterized using high throughput instrumentation which confirms the generation of stable, crystalline XsAgNPs and XsAuNPs with the mean size of 14 ± 6 and 14 ± 5, respectively. Further, the NPs exhibits an excellent bactericidal effect against six different pathogens. On the other hand, NPs displayed an outstanding anticancer activity against human lung adenocarcinoma epithelial cells (A549). Therefore, the present study strongly suggests that metal NPs encrusted with functional bio-moieties can be used for different biomedical applications.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1080/21691401.2017.1282495 | DOI Listing |
Molecules
June 2024
División de Estudios de Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Santiago de Querétaro 76010, Querétaro, Mexico.
Secondary metabolites, bioactive compounds produced by living organisms, can unveil symbiotic relationships in nature. In this study, soilborne entomopathogenic nematodes associated with symbiotic bacteria ( and ) were extracted from solvent supernatant containing secondary metabolites, demonstrating significant inhibitory effects against , , , , , and . The characterization of these secondary metabolites by Fourier transforms infrared spectroscopy revealed amine groups of proteins, hydroxyl and carboxyl groups of polyphenols, hydroxyl groups of polysaccharides, and carboxyl groups of organic acids.
View Article and Find Full Text PDFWorld J Microbiol Biotechnol
February 2024
State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Science, Hunan Normal University, No.36 Lushan Street, Changsha, 410081, China.
Xenorhabdus, known for its symbiotic relationship with Entomopathogenic nematodes (EPNs), belongs to the Enterobacteriaceae family. This dual-host symbiotic nematode exhibits pathogenic traits, rendering it a promising biocontrol agent against insects. Our prior investigations revealed that Xenorhabdus stockiae HN_xs01, isolated in our laboratory, demonstrates exceptional potential in halting bacterial growth and displaying anti-tumor activity.
View Article and Find Full Text PDFFront Bioeng Biotechnol
September 2022
Helmholtz International Lab for Anti-infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
are symbionts of soil entomopathogenic nematodes of the genus presenting two distinct forms in their life cycle, and can produce a broad range of bioactive compounds. In this study, a novel strain HN_xs01 was isolated from a soil sample via an entrapment method using nematodes. The supernatants of strain HN_xs01 exhibited antimicrobial properties against Gram-negative and Gram-positive bacteria, and insecticidal properties against larvae, and antitumor properties as well.
View Article and Find Full Text PDFTrop Biomed
September 2020
Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, India.
To characterize the production and larvicidal activity of Xenorhabdus stockiae KUT6 Petroleum ether extracts from Luria Broth and induced Quorum sensing medium containing N-3- oxododecanoyl Homoserine Lactone inducer against dengue vector Aedes aegypti. The Galleria mellonella larvae were reared for the isolation of Steinernema saimkayi symbiont Xenorhabdus stockiae KUT6 from Cucumber field soil sample in NBTA. Then for the extraction of compounds the KUT6 strains were cultured in Luria Broth and Quorum Sensing optimized media using N-3-oxododecanoyl homoserine lactone inducer.
View Article and Find Full Text PDFJ Helminthol
September 2020
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.
Entomopathogenic nematodes (EPNs) Steinernema and Heterorhabditis and their symbiotic bacteria, Xenorhabdus and Photorhabdus, have been successfully used for the control of insect pests. The objectives of this study were to survey the EPNs and symbiotic bacteria in the agricultural areas of the Phitsanulok province, Thailand, and to study the association between the soil parameters and presence of EPNs. We collected 200 soil samples from 40 soil sites in agricultural areas (field crops, horticulture crops and forest).
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!