A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine.

Diagn Microbiol Infect Dis

Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz/FIOCRUZ, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, Brazil. Electronic address:

Published: April 2017

Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for bla, bla and bla and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.

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http://dx.doi.org/10.1016/j.diagmicrobio.2017.01.005DOI Listing

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