AI Article Synopsis
- The study utilizes Hain GenoType MTBDR assays to uncover that certain mutations can lead to incorrect resistance results for fluoroquinolones, impacting both wild-type and mutant probe binding.
- These mutations, while rare worldwide, are present in about 7% of multidrug-resistant tuberculosis strains in specific areas.
- This finding highlights the need for careful interpretation of assay results in TB treatment to ensure accurate resistance detection.
Article Abstract
In this study, using the Hain GenoType MTBDR assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in in result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5365657 | PMC |
| http://dx.doi.org/10.1128/AAC.02169-16 | DOI Listing |
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