Permanent draft genome of DSM 505, an obligately chemolithoautotrophic member of the .

DSM 505 is one of first two isolated strains of inorganic sulfur-oxidising . The original strain of was lost almost 100 years ago and the working type strain is Culture C (=DSM 505 = ATCC 8158) isolated by Starkey in 1934 from agricultural soil at Rutgers University, New Jersey, USA. It is an obligate chemolithoautotroph that conserves energy from the oxidation of reduced inorganic sulfur compounds using the Kelly-Trudinger pathway and uses it to fix carbon dioxide It is not capable of heterotrophic or mixotrophic growth. The strain has a genome size of 3,201,518 bp. Here we report the genome sequence, annotation and characteristics. The genome contains 3,135 protein coding and 62 RNA coding genes. Genes encoding the transaldolase variant of the Calvin-Benson-Bassham cycle were also identified and an operon encoding carboxysomes, along with Smith's biosynthetic horseshoe of Krebs' cycle . Terminal oxidases were identified, . cytochrome oxidase (3, EC 1.9.3.1) and ubiquinol oxidase (, EC 1.10.3.10). There is a partial operon of the Kelly-Friedrich pathway of inorganic sulfur-oxidation that contains genes but lacking , there is also a lack of the DUF302 gene previously noted in the operon of other members of the '' that can use trithionate as an energy source. In spite of apparently not growing anaerobically with denitrification, the , , and operons encoding enzymes of denitrification are found in the genome, in the same arrangements as in the true denitrifier .