Objective: Acute otitis media (AOM) causes an inflammatory response in the middle ear. We assessed differences in innate immune responses involved in bacterial defense at onset of AOM in children who were stringently defined as otitis prone (sOP) and children not otitis prone (NOP).
Study Design: Innate immune genes analysis from middle ear fluid (MEF) samples of children.
Methods: Genes of toll-like receptors (TLR), nod-like and retinoic acid-inducible gene-I-like receptors, downstream effectors important for inflammation and apoptosis, including cytokines and chemokines, were studied from MEF samples by using a real-time polymerase chain reaction array. Protein levels of differentially regulated genes were measured by Luminex.
Results: Gene expression in MEF among children who were sOP was significantly different in upregulation of interleukin 8, secretory leukocyte peptidase inhibitor, and chemokine (C-C motif) ligand 3, and in downregulation of interferon regulatory factor 7 and its related signaling molecules interferon alpha, Toll-like receptor adaptor molecule 2, chemokine (C-C motif) ligand 5, and mitogen-activated protein kinase 8 compared with children who were NOP. Differences in innate gene regulation were similar when AOM was caused by Streptococcus pneumoniae or nontypeable Haemophilus influenzae.
Conclusion: Innate-immune response genes are differentially regulated in children who were sOP compared with children with NOP.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108842 | PMC |
| http://dx.doi.org/10.2500/ajra.2016.30.4393 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Serous otitis media as a cause for language delay following cleft palate repair-A case-control study.
Natl J Maxillofac Surg
November 2024
Department of ENT, Jubilee Mission Medical College and Research Institute, Thrissur, Kerala, India.
Background And Objectives: Serous otitis media (SOM), also called otitis media with effusion (OME) or glue ear, is a collection of non-purulent fluid within the middle ear space. Children with cleft palate are more prone to develop this condition. This is caused by impaired eustachian tube function in cleft palate.
View Article and Find Full Text PDFRole as a Probiotic in Children's Health and Disease Prophylaxis-A Systematic Review.
Life (Basel)
December 2024
Physiopathology Department, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology, 540142 Targu Mures, Romania.
Background: This systematic review aimed to synthesize the existing evidence on the use of () probiotics as prophylactic or therapeutic tools for pediatric oral, dental, and respiratory diseases.
Methods: A comprehensive search was carried out across multiple databases using the following terms: , probiotic, children, pediatric.
Results: The systematic literature search identified 613 publications, which were meticulously screened, and, ultimately, 15 suitable citations were included in this systematic review.
Bromelain Supplementation in the Management of Otitis Media with Effusion in Children.
Children (Basel)
November 2024
Department of Health Promotion Sciences, Maternal and Child Care, Internal Medicine and Medical Specialties 'Giuseppe D'Alessandro', University of Palermo, Via del Vespro 133, 90127 Palermo, Italy.
Background/objectives: The respiratory system is prone to infectious diseases, especially in children below five years of age. Upper respiratory tract infections in children are often associated with Eustachian tube dysfunction and complicated by otitis media with effusion (OME), an inflammatory process within the middle ear, which can lead to hearing loss. Treatment for these infections involves a combination of medication and symptom relief, depending on the severity and cause of the infection.
View Article and Find Full Text PDFThe long-term efficacy and safety of balloon dilation eustachian tuboplasty combined with tympanostomy tube insertion for patients with otitis media with effusion: study protocol for a prospective randomized controlled trial.
Trials
August 2024
Department of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, Shanghai, China.
Article Synopsis
- Otitis media with effusion (OME) is commonly caused by eustachian tube dysfunction and leads to problems like hearing loss; traditional treatment with tympanostomy tube insertion (TTI) alleviates symptoms but is not a definitive cure.
- The study aims to assess the effectiveness of a new procedure, balloon dilation eustachian tuboplasty (BDET), in combination with TTI in patients with OME through a randomized controlled trial involving 124 participants.
- Outcomes will be measured over two years using various assessments to evaluate eustachian tube function, middle ear function, hearing improvement, and quality of life, marking an important investigation into the long-term benefits and safety of this combined treatment.
Relationship between sleep position and otitis media in infants: The Japan environment and children's study.
Auris Nasus Larynx
October 2024
Fukushima Regional Center for the Japan Environment and Children's Study, Fukushima, Japan; Department of Otolaryngology, Fukushima Medical University School of Medicine, Fukushima, Japan.
Article Synopsis
- The study explores the link between sleep position and the occurrence of otitis media in infants, highlighting that sleep position may influence health outcomes.
- Data from the Japan Environment Children's Study involving 85,731 infants were analyzed, revealing a higher incidence of otitis media when infants slept in the prone position.
- The findings suggest that promoting safe sleep positions could help prevent otitis media among infants under 6 months old.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!